ОПЫТ КАФЕДРЫ «БИОНАНОТЕХНОЛОГИЯ» КЕМЕРОВСКОГО ТЕХНОЛОГИЧЕСКОГО ИНСТИТУТА ПИЩЕВОЙ ПРОМЫШЛЕННОСТИ В ОБЛАСТИ БИОТЕХНОЛОГИИ ПОЛУЧЕНИЯ РЕКОМБИНАНТНЫХ ФЕРМЕНТНЫХ ПРЕПАРАТОВ

EXPERIENCE OF THE DEPARTMENT OF «BIONANOTECHNOLOGY» OF THE KEMEROVO INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY IN THE FIELD OF BIOTECHNOLOGY OF OBTAINING RECOMBINANT ENZYME PREPARATIONS

Alexander Yu. Prosekov ^a

Olga O. Babich ^b

Soldatova L. S. ^c

Affiliation

a Kemerovo State University

b Immanuel Kant Baltic Federal University

c Kemerovo Institute of Food Science and Technology

Copyright ©Prosekov et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0. (http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.

Abstract

The review of modern methods of obtaining recombinant enzymes for food industry is given. Optimum condi-tions to obtain recombinant L-phenylalanine-ammonia-lyase Rhodosporidium toruloides allocated from Escherichia coli have been established. The share of the special-purpose protein obtained after the induction was 40 % of the total cell protein. The clearing of the obtained protein on Ni-NTA agarose has been done. The degree of protein clearing was more than 90 %.

Keywords

Recombinant strain, enzyme, cloning, genetic engineering, construction, L-phenylalanine-ammonia-lyase, trans-formation, expression vector.

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