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Title of article CLONAL MICROPROPAGATION OF CRANBERRY (OXYCOCCUS PALUSTRIS PERS.)
Authors

Makarov S., Central European Forest Experimental Station, Kostroma, Russia, makarov_serg44@mail.ru

Kuznetsova I., Kostroma State Agricultural Academy, Karavaevo, Russia

Upadyshev M., Federal Horticultural Research Center for Breeding, Agrotechnology and Nursery, Moscow, Russia

Rodin S. , All-Russian Scientific Research Institute of Silviculture and Mechanization of Forestry, Pushkino, Russia

Chudetsky A., Central European Forest Experimental Station, Kostroma, Russia

Section
Year 2021 Issue 1 UDC 634.739.2
DOI 10.21603/2074-9414-2021-1-67-76
Abstract Introduction. The last decade saw a considerable increase in the demand for European cranberry planting material (Oxyccocus palustris Pers.) among consumers of non-timber forest products. Cranberry possesses high nutritional and medicinal value. Cultivars and hybrids of European cranberry prove extremely productive for plantation growth using the method of clonal micropropagation with revitalized planting material.
Study objects and methods. The research featured European cranberry plants of the Dar Kostromy cultivar and its hybrid form 1-15-635. The study focused on the effect of various medications and growth regulators on the biometric profile of European cranberry and its adaptation to non-sterile conditions at all stages of in vivo clonal micropropagation.
Results and discussion. During the introduction stage, the highest viability belonged to the explants treated with AgNO3 (95–96%) and Lizoformin 3000 (5%) as the main sterilizing solutions at a 10-min exposure and a 5% solution of Ecosterilizer (1:1) at a 20-min exposure (90–95%). During the micropropagation proper, the number, average length, and total growth of shoots increased as the concentration of cytokinin 2ip in the WPM 1/4 nutrient medium rose from 1.0 to 5.0 mg/L. At the stage of in vitro rooting, the maximal number, average length, and total growth of roots in regenerated plants for both cultivars were observed when Kornerost 5.0 mg/L was added to the WPM 1/4 nutrient medium. At the stage of adaptation to in vivo conditions, Micogel 0.2 mg/L contributed to the highest survival rate (94–100%).
Conclusion. During clonal micropropagation in vitro, the biometric profile of European cranberry (Oxyccocus palustris Pers.) and its survival rate under non-sterile conditions in vivo proved to depend on various growth-regulating substances and their concentrations.
Keywords European cranberry, clonal micropropagation, in vitro, in vivo, sterilizing solution, adaptation
Artice information Received January 18, 2021
Accepted February 15, 2021
Available online March 25, 2021
For citation Makarov SS, Kuznetsova IB, Upadyshev MT, Rodin SA, Chudetsky AI. Clonal Micropropagation of Cranberry (Oxycoccus palustris Pers.). Food Processing: Techniques and Technology. 2021;51(1):67–76. (In Russ.). https://doi. org/10.21603/2074-9414-2021-1-67-76.
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References
  1. Makarov SS, Bagayev ES, Tsaregradskaya SYu, Kuznetsova IB. Problems of use and reproduction of phytogenic food and medicinal forest resources on the forest fund lands of the Kostroma region. Lesnoy Zhurnal (Russian Forestry Journal). 2019;372(6):118–131. (In Russ.). https://doi.org/10.17238/issn0536-1036.2019.6.118.
  2. Korenev IA, Tyak GV, Makarov SS. Creation of new varietiesof forest berry plantsand prospects of their intensive reproduction (in vitro). Forestry Information. 2019;(3):180–189. (In Russ.). https://doi.org/10.24419/LHI.2304-3083.2019.3.15.
  3. Oszmiański J, Wojdiło A, Lachowicz S, Gorzelany J, Matłok N. Comparison of bioactive potential of cranberry fruit and fruit-based products versus leaves. Journal of Functional Foods. 2016;22:232–242. https://doi.org/10.1016/j.jff.2016.01.015.
  4. Karlsons A, Osvalde A, Čekstere G, Ponnale J. Research on the mineral composition of cultivated and wild blueberries and cranberries. Agronomy Research. 2018;16(2):454–463. https://doi.org/10.15159/AR.18.039.
  5. Gu K-D, Wang C-K, Hu D-G, Hao Y-J. How do anthocyanins paint our horticulture products? Scientia Horticulturae. 2019;249:257–262. https://doi.org/10.1016/j.scienta.2019.01.034.
  6. Peron G, Sut S, Pellizzaro A, Brun P, Voinovich D, Castagliuolo I, et al. The antiadhesive activity of cranberry phytocomplex studied by metabolomics: Intestinal PAC-A metabolites but not intact PAC-A are identified as markers in active urines against uropathogenic Escherichia coli. Fitoterapia. 2017;122:67–75. https://doi.org/10.1016/j.fitote.2017.08.014.
  7. Debnath SC, An D. Antioxidant properties and structured biodiversity in a diverse set of wild cranberry clones. Heliyon. 2019;5(4). https://doi.org/10.1016/j.heliyon.2019.e01493.
  8. Philip N, Walsh LJ. Cranberry polyphenols: Natural weapons against dental caries. Dentistry Journal. 2019;7(1). https:// doi.org/10.3390/dj7010020.
  9. Rocha DMUP, Caldas APS, da Silva BP, Hermsdorff HHM, Alfenas RDCG. Effects of blueberry and cranberry consumption on type 2 diabetes glycemic control: A systematic review. Critical Reviews in Food Science and Nutrition. 2019;59(11):1816–1828. https://doi.org/10.1080/10408398.2018.1430019.
  10. Coleman CM, Ferreira D. Oligosaccharides and complex carbohydrates: A new paradigm for cranberry bioactivity. Molecules. 2020;25(4). https://doi.org/10.3390/molecules25040881.
  11. Matsneva OV, Tashmatova LV. Clonal micro-propagation of strawberries is a promising method of modern nursery practice (review). Contemporary Horticulture. 2019;4:113–119. (In Russ.). https://doi.org/10.24411/2312-6701-2019-10411.
  12. Sedlák J, Paprštein F. Micropropagation of cranberry (Vaccinium macrocarpon) through shoot tip cultures – Short communication. Horticultural Science. 2011;38(4):159–162. https://doi.org/10.17221/115/2010-HORTSCI.
  13. Litwińczuk W. Micropropagation of Vaccinium sp. by in vitro axillary shoot proliferation. In: Lambardi M, Ozudogru EA, Jain SM, editors. Protocols for micropropagation of selected economically-important horticultural plants. Totowa: Humana Press; 2013. pp. 63–76. https://doi.org/10.1007/978-1-62703-074-8_5.
  14. Zontikov DN, Zontikova SA, Malakhova KV, Maramokhin EV. Influence of the composition of nutritional media and growth regulators during clonal micropropagation of some polyliploid forms of the genus Vaccinium L. Izvestia of Samara Scientific Center of the Russian Academy of Sciences. 2019;21(2)(88):39–44. (In Russ.).
  15. Yang Y, Asyakina LK, Babich OO, Dyshlyuk LS, Sukhikh SA, Popov AD, et al. Physicochemical properties and biological activity of extracts of dried biomass of callus and suspension cells and in vitro root cultures. Food Processing: Techniques and Technology. 2020;50(3):480–492. (In Russ.). https://doi.org/10.21603/2074-9414-2020-3-480-492.