Abstract

Introduction. Modern food science needs new research of food emulsifiers, their composition, properties and effect on the structural characteristics of emulsions. It looks for modern technological solutions on how to select proper emulsifiers and their mixes to produce emulsions with different mass fractions of fat. The research objective was to study the effect of physical and chemical indicators of surfactants on the properties of food emulsions, as well as to develop practical recommendations for the selection of surfactants for various types of products.
Study objects and methods. The research featured model dairy fat emulsions and laboratory-made vegetable oil, as well as hard and soft mono- and diglycerides of fatty acids and lecithins. The emulsifiers were used to determine the melting point, fatty acid composition, iodine number, and solid triglyceride content at various temperatures. The melting point of emulsifiers was determined by fixing the melting temperature in a capillary oven. To identify the fatty acid composition, the methyl esters of fatty acids were subjected to the chromatogram method. After that, the separated components and their quantity were determined by the area of the peaks. The content of solid triglycerides in the emulsifiers was determined by the method of nuclear magnetic resonance. The hydrophilic-lipophilic balance was obtained from the manufacturer's specifications.
Results and discussion. The solid mono- and diglycerides appeared to have a high content of stearic and palmitic acids. Oleic acid predominated in soft monoglycerides; unsaturated fatty acids (linolenic and oleic) also predominated in the monoglycerides/lecithin complex emulsifier. Solid monoglycerides had a high content of solid triglycerides at 35°C (82.93%), which correlated with the high melting point (80°C) and the lowest iodine number (3 mg I₂/100 g) of all the samples. The optimal ratio of vegetable oil and the emulsifier was defined empirically. The emulsifiers were dissolved in refined deodorized vegetable oil at 5–7°C above the melting point of the emulsifier. The resulting ratios were between 6:1 and 10:1. The samples of creamy vegetable spreads were obtained using the studied emulsifiers and their compositions in different doses and ratios. The crystallization temperature and phase transition time were determined when studying the process of emulsion overcooling. The article introduces a list of technological and physicochemical indicators of emulsifiers: the fatty acid composition, the degree of saturation, the melting point, and the content of solid triglycerides. By finding out the physicochemical parameters of emulsifiers, producers can vary the ratio of the components of emulsifying compositions to achieve the desired properties of food emulsions. The hydrophilic-lipophilic balance also proved to be an important index since the proportion of hydrophilic and hydrophobic groups in surfactants affects the type of emulsions and makes it possible to adjust the fat content of the finished product.
Conclusion. The research results can expand the theoretical foundations of food emulsions. The article contains scientifically grounded recommendations on how to select optimal surfactants. The research opens up prospects for further studies of emulsifiers and their effect on the quality of finished products.

Keywords

Emulsifiers, hydrophilic-lipophilic balance, surface-active properties, coagulation structures, crystallization structures, fatty acid composition

REFERENCES

1. Tarasova LI, Tagijeva TG, Zavadskaja IM. To the question of evaluation of food SAS efficiency for the emulsion products of oil and fat product range. Vestnik of the All-Russia Scientific Research Institute of Fats. 2019;(1–2):39–43. (In Russ.).
2. Tereshchuk LV, Starovoytova KV, Ivashina OA. Practical aspects of the use of emulsifiers in manufacturing emulsion fat-and-oil products. Foods and Raw Materials. 2018;6(1):30–39. https://doi.org/10.21603/2308-4057-2018-1-30-39.
3. Topnikova EV, Lepilkina OV, Konopleva AA. Vliyanie ehmulʹgatorov na reologicheskie svoystva spredov [Effect of emulsifiers on the rheological properties of spreads]. Milk Processing. 2014;179(9):44–46. (In Russ.).
4. Fredrick E, Heyman B, Moens K, Fischer S, Verwijlen T, Moldenaers P, et al. Monoacylglycerols in dairy recombined cream: II. The effect on partial coalescence and whipping properties. Food Research International. 2013;51(2):936–945. https://doi.org/10.1016/j.foodres.2013.02.006.
5. Fredrick E, Walstra P, Dewettinck K. Factors governing partial coalescence in oil-in-water emulsions. Advances in Colloid and Interface Science. 2010;153(1–2):30–42. https://doi.org/10.1016/j.cis.2009.10.003.
6. Petrut RF, Danthine S, Blecker C. Assessment of partial coalescence in whippable oil-in-water food emulsions. Advances in Colloid and Interface Science. 2016;229:25–33. https://doi.org/10.1016/j.cis.2015.12.004.
7. Prosekov AYu, Dyshlyuk LS, Milent'eva IS, Pavsky VA, Ivanova SA, Garmashov SYu. Study of the biofunctional properties of cedar pine oil with the use of in vitro testing cultures. Foods and Raw Materials. 2018;6(1):136–143. https://doi.org/10.21603/2308-4057-2018-1-136-143.
8. Ali F, Wang J, Ullah N. Oil/fat blending strategy for improving milk fat globule membrane stability and its effect on fatty acid composition. International Journal of Dairy Technology. 2019;72(4):496–504. https://doi.org/10.1111/1471-0307.12604.
9. Habashi V, Elhamirad AH, Pedramnia A. Textural properties of low fat mayonnaise with whey protein concentrate and Tragacanth gum as egg and fat substitutes. Foods and Raw Materials. 2021;9(1):19–23. https://doi.org/10.21603/2308-4057-2021-1-19-23.
10. Tippetts M, Martini S. Effect of cooling rate on lipid crystallization in oil-in-water emulsions. Food Research International. 2009;42(7):847–855. https://doi.org/10.1016/j.foodres.2009.03.009.
11. Tavernier I, Moens K, Heyman B, Danthine S, Dewettinck K. Relating crystallization behavior of monoacylglycerols-diacylglycerol mixtures to the strength of their crystalline network in oil. Food Research International. 2019;120:504–513. https://doi.org/10.1016/j.foodres.2018.10.092.
12. Moens K, Tzompa-Sosa DA, Van de Walle D, Van der Meeren P, Dewettinck K. Influence of cooling rate on partial coalescence in natural dairy cream. Food Research International. 2019;120:819–828. https://doi.org/10.1016/j.foodres.2018.11.044.
13. Loi CC, Eyres GT, Birch EJ. Effect of mono- and diglycerides on physical properties and stability of a protein-stabilised oil-in-water emulsion. Journal of Food Engineering. 2019;240:56–64. https://doi.org/10.1016/j.jfoodeng.2018.07.016.
14. Moens K, Tavernier I, Dewettinck K. Crystallization behavior of emulsified fats influences shear-induced partial coalescence. Food Research International. 2018;113:362–370. https://doi.org/10.1016/j.foodres.2018.07.005.
15. Doğan M, Göksel Saraç M, Aslan Türker D. Effect of salt on the inter-relationship between the morphological, emulsifying and interfacial rheological properties of O/W emulsions at oil/water interface. Journal of Food Engineering. 2018;275. https://doi.org/10.1016/j.jfoodeng.2019.109871.
16. Blankart M, Kratzner C, Link K, Oellig C, Schwack W, Hinrichs J. Technical emulsifiers in aerosol whipping cream – Compositional variations in the emulsifier affecting emulsion and foam properties. International Dairy Journal. 2020;102. https://doi.org/10.1016/j.idairyj.2019.104578.
17. Jadhav H, Waghmare J, Annapure U. Effect of mono and diglyceride of medium chain fatty acid on the stability of flavour emulsion. Food Research. 2021;5(2):214–220. https://doi.org/10.26656/fr.2017.5(2).589.
18. Katsouli M, Tzia C. Effect of lipid type, dispersed phase volume fraction and emulsifier on the physicochemical properties of nanoemulsions fortified with conjugated linoleic acid (CLA): Process optimization and stability assessment during storage conditions. Journal of Molecular Liquids. 2019;292. https://doi.org/10.1016/j.molliq.2019.111397.
19. Maherani B, Khlifi MA, Salmieri S, Lacroix M. Design of biosystems to provide healthy and safe food. Part A: effect of emulsifier and preparation technique on physicochemical, antioxidant and antimicrobial properties. European Food Research and Technology. 2018;244(11):1963–1975. https://doi.org/10.1007/s00217-018-3108-2.
20. Marhamati M, Ranjbar G, Rezaie M. Effects of emulsifiers on the physicochemical stability of oil-in-water nanoemulsions: A critical review. Journal of Molecular Liquids. 2021;340. https://doi.org/10.1016/j.molliq.2021.117218.
21. Okuro PK, Gomes A, Costa ALR, Adame MA, Cunha RL. Formation and stability of W/O-high internal phase emulsions (HIPEs) and derived O/W emulsions stabilized by PGPR and lecithin. Food Research International. 2019;122:252–262. https://doi.org/10.1016/j.foodres.2019.04.028.
22. Oppermann AKL, Noppers JME, Stieger M, Scholten E. Effect of outer water phase composition on oil droplet size and yield of (w1/o/w2) double emulsions. Food Research International. 2018;107:148–157. https://doi.org/10.1016/j.foodres.2018.02.021.
23. Pathak M. Nanoemulsions and their stability for enhancing functional properties of food ingredients. In: Oprea AE, Grumezescu AM, editors. Nanotechnology applications in food: Flavor, stability, nutrition and safety. Academic Press; 2017. pp. 87–106. https://doi.org/10.1016/B978-0-12-811942-6.00005-4.
24. Soo YN, Tan CP, Tan PY, Khalid N, Tan TB. Fabrication of oil-in-water emulsions as shelf-stable liquid non-dairy creamers: Effects of homogenization pressure, oil type, and emulsifier concentration. Journal of the Science of Food and Agriculture. 2021;101(6):2455–2462. https://doi.org/10.1002/jsfa.10871.
25. Novoselova MV, Prosekov AYu. Technological options for the production of lactoferrin. Foods and Raw Materials. 2016;4(1):90–101. https://doi.org/10.21179/2308-4057-2016-1-90-101.
26. Yildirim M, Sumnu G, Sahin S. The effects of emulsifier type, phase ratio, and homogenization methods on stability of the double emulsion. Journal of Dispersion Science and Technology. 2017;38(6):807–814. https://doi.org/10.1080/01932691.2016.1201768.
27. Zheng H, Mao L, Yang J, Zhang C, Miao S, Gao Y. Effect of oil content and emulsifier type on the properties and antioxidant activity of sea buckthorn oil-in-water emulsions. Journal of Food Quality. 2020;2020. https://doi.org/10.1155/2020/1540925.
28. Sun Y, Liu Z, Wang X, Zhang F, Huang X, Li J, et al. Effect of HLB value on the properties of chitosan/zein/lemon essential oil film-forming emulsion and composite film. International Journal of Food Science and Technology. 2021;56(10):4925–4933. https://doi.org/10.1111/ijfs.15216.