ISSN 2074-9414 (Print),
ISSN 2313-1748 (Online)

The composition and structure of anthocyanins in wines produced from early varieties of grapes grown in the Altai Territory

Abstract
Different ways were used to produce wines from early varieties of grapes cultivated in the Altai territory. The peculiarity of wine production was the use of ultrasonic and enzymatic treatment of the grape pulp. Dry wines had significant visual differences. The color intensity of wine was increased by enzymatic treatment of pulp. Enzymatic hydrolysis was carried out using Rapidase CR preparation. Ultrasonic treatment of pulp contributed to accumulation of polyphenols and anthocyanins. Ultrasonic treatment of pulp before fermentation considerably influenced the quantity of monomeric fraction of anthocyanins. To carry out ultrasonic treatment the apparatus with the frequency of generated ultrasonic vibrations of 22000 ± 1650 Hz and the radiation intensity of 150000 W/m 2 was used. Two variants of ultrasonic treatment of original pulp were studied. The sample produced by a blend of grapes, with a low content of phenol and coloring substances, had high color intensity. The taste evaluation of dry wines was done. The proportion of coloring substances in wines was found. The basic anthocyanin of grape was identified. The anthocyanin was isolated with one-dimensional ascending paper chromatography. The mobile phase used was n-butanol: acetic acid: water (40:12:29). The chromatogram had six areas, but one of them was dominant. The chromatogram had two areas with significantly different Rf when carrying acid hydrolysis of anthocyanin. The mass spectrum showed the ion 493 m/z [M] corresponding to the molecular weight of malvidin-3-O-glucoside. Ion 509 m/z, formed by pseudobase of malvidin monoglucoside was found in the negative region of the mass spectrum. When recording NMR for substance discolving CH 3OD + CF 3COOH was used. NMR spectrum of the medium coincided with the literature data for malvidin-3-O-glucoside.
Keywords
Grape, wine, color, anthocyanins, paper chromatography, NMR, malvidin-3-O-glucoside
REFERENCES
  1. Vagner, V.A. Issledovanie francuzskih sortov vinograda, proizrastayuschih na territorii OAO «Altayskiy vinzavod» / V.A. Vagner, Yu.I. Yusupova // Sovremennye problemy tehniki i tehnologii pischevyh proizvodstv: 14-ya Mezhdunar. nauchno-prakt. konf., g. Barnaul, 29 noyabrya 2013 g. - Barnaul, 2013. - 261 s.
  2. Makarova, G.A. Hozyaystvenno-biologicheskaya ocenka sortov vinograda v usloviyah umerenno zasushlivoy i kolochnoy stepi Altayskogo kraya: dis.. kand. s.-h. nauk: 06.01.07 / Makarova Galina Aleksandrovna. - Barnaul, 2007. - 174 s.
  3. Sharov, R.F. Spravochnaya kniga vinogradarya Sibiri / R.F. Sharov. - Barnaul: Altayskaya pravda, 1991. - 157 s.
  4. Aparneva, M.A. Tehnologicheskaya ocenka krasnyh sortov vinograda, kul'tiviruemyh na Altae / M.A. Aparneva, V.P. Sevodin // Tehnika i tehnologiya pischevyh proizvodstv. - 2013. - № 2. - S. 107-111.
  5. Pechenina, A.A. Ocenka kachestva belyh sortov vinograda, kul'tiviruemyh na Altae / A.A. Pechenina, V.P. Sevodin // Tehnika i tehnologiya pischevyh proizvodstv. - 2013. - № 3. - S. 129-132.
  6. Aparneva, M.A. Kachestvo vinnyh napitkov iz krasnyh sortov vinograda Altayskogo kraya / M.A. Aparneva, V.P. Sevodin // Tehnika i tehnologiya pischevyh proizvodstv. - 2013. - № 4. - S. 31-34.
  7. Skorikova, Yu.G. Polifenoly plodov i ovoschey i formirovanie cveta produktov / Yu.G. Skorikova. - M.: Pischevaya promyshlennost', 1973. - 232 s.
  8. Kishkovskiy, Z.N. Himiya vina / Z.N. Kishkovskiy, I.M. Skurihin. - M.: Pischevaya promyshlennost', 1976. - 312 s.
  9. Smirnov, E.V. Pischevye krasiteli. Spravochnik / E.V. Smirnov. - SPb.: Professiya, 2009. - 352 s.
  10. Wrolstad, R.E. Current protocols in food analytical chemistry / R.E. Wrolstad // Food Analytical Chemistry. - N. Y.: John Wiley & Sons Inc., 2001.
  11. Skorbanova, E.A. Opredelenie chistosortnosti vin iz vinograda Vitis vinifera / E.A. Skorbanova, P.D. Rynda, N.F. Kayryak // Vinodelie i vinogradarstvo. - 2006. - № 1. - S. 24-25.
  12. Timberlake, F.C. Anthocyanins - occurrence, extraction and chemistry / F.C. Timberlake // Food Chemistry. - 1980. - № 5. - R. 69-80.
  13. Ul'trazvukovye mnogofunkcional'nye i specializirovannye apparaty dlya intensifikacii tehnologicheskih processov v promyshlennosti / V.N. Hmelev i dr. - Barnaul: AltGTU, 2007. - 400 s.
  14. Valuyko, G.G. Identifikaciya antocianov vinograda / G.G. Valuyko, L.M. Germanova // Vinodelie i vinogradarstvo SSSR. - 1969. - № 6. - S. 19-25.
  15. Tanchev, S.S. Antociany v plodah i ovoschah / S.S. Tanchev. - M.: Pischevaya promyshlennost', 1980. - 304 s.
  16. Metody tehnohimicheskogo kontrolya v vinodelii / pod red. V.G. Gerzhikovoy. - Simferopol': Tavrida, 2002. - 260 s.
  17. Sbornik mezhdunarodnyh metodov analiza i ocenki vin i susel / pod red. N.A. Mehuzla. - M.: Pischevaya promyshlennost', 1993. - 319 s.
  18. Bakker, J. Isolation, Identification, and Characterization of New Color-Stable Anthocyanins Occurring in Some Red Wines / J. Bakker, C.F. Timberlake // J. Agric. Food Chem. - 1997. - № 45. - R. 35-43.
  19. Cheminat, A. PMR investigation of 3-O-(-D-glucosyl)malvidin structural transformations in aqueous solutions / A. Cheminat, R. Brouillard // Tetrahedron Letters. - 1986. - № 27. - R. 4457-4460.
  20. Pale, E. Anthocyanins from Bambara Groundnut (Vigna subterranea) / E. Pale, M. Nacro, M. Vanhaelen, R. Vanhaelen-Fastre // Journal of Agricultural and Food Chemistry. - 1997. - № 45. - R. 3359-3361.
  21. Mas, T. DNA triplex stabilization property of anthocyanins / T. Mas, J. Susperregui, B. Berke et al. // Phytochemistry. - 2000. - № 53. - R. 679-687.
  22. Vydelenie i ochistka antocianov vinograda Vitis vinifera L. sorta Izabella / A.V. Pticyn, A.P. Kaplun, E.I. Muhtarov, V.I. Shvec // Biotehnologiya. - 2007. - № 2. - S. 13-20.
  23. Atanasova, V. Structure of a new dimeric acetaldehyde malvidin 3-glucoside condensation product / V. Atanasova, H. Fulcrand, C.L. Guerneve et al. // Tetrahedron Letters. - 2002. - № 48. - R. 6151-6153.
  24. Pedersen, A.T. NMR of anthocyanins: assignments and effects of exchanging aromatic protons / A.T. Pedersen, O.M. Andersen, D.W. Aksnes, W. Nerdal // Magnetic resonance in chemistry. - 1993. - № 31. - R. 972-976.
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