ISSN 2074-9414 (Print),
ISSN 2313-1748 (Online)

Trace Elements in the Meat and Internal Organs of the Mountain Hare (Lepus timidus L., 1758) in the North of the Krasnoyarsk Region

Abstract
Relevant data on trace elements and toxic metals in game meat make it possible to monitor chemical pollution, as well as to detect risks to human and animal health.
The authors used atomic absorption spectrometry to study iron, copper, zinc, lead, and cadmium in the skeletal muscles, liver, and kidneys of mountain hares (Lepus timidus L.) (n = 107). The animals were caught during two seasons on reference sites and technogenic territories in the northern Krasnoyarsk Region.
The samples obtained from the hares that lived on the reference sites had a much lower content of toxic metals. The tissues that belonged to the hares from the polluted habitats contained more lead and cadmium while the samples from the reference sites demonstrated traces of such biogenic elements as copper, zinc, and iron. The differences may be associated with the antagonism of biogenic elements and toxic metals in the body. The correlations between various metals probably meant that they came from one and the same pollution source.
The levels of cadmium and lead in the samples from the technogenic areas were not hazardous to animal health. However, their meat and liver were unfit to eat. Therefore, hunting in the areas of technogenic pollution can pose a toxic hazard to indigenous hunters and their families.
Keywords
Lepus timidus, mountain hare, hunting, game meat, pollution, ecotoxicology, trace elements, heavy metals, lead, cadmium, mercury
Contribution
All the authors contributed equally to the study and bear equal responsibility for information published in this article.
CONFLICTS OF INTEREST
The authors declare that there is no conflict of interests regarding the publication of this article.
ACKNOWLEDGEMENTS
The authors express their sincere gratitude to indigenous hunters A.N. Betu, N.N. Porotov, and Z.I. Veselovskaya, as well as the team of the Reference Center of the Krasnoyarsk Federal Rosselkhoznadzor Service, who helped to process the biological material.
FUNDING
The research was conducted on the premises of the Professor Zhitkov Russian Research Institute of Game Management and Fur Farming (VNIIOZ) as part of the State Task on Scientific Foundations for Sustainable Use, Assessment, Monitoring, and Forecasting Biological Resources of the Hunting Industry (No. FNWS-2022-0001), Fundamental Research Program through 2021–2030, Decree No. 3684-r of the Government of the Russian Federation, December 31, 2020.
REFERENCES
  1. Bremmer I. Topics in environmental health. Research and Reviews: Journal of Veterinary Sciences. 1979;2(1):2016–2020.
  2. Celechovská O, Malota L, Zima S. Entry of heavy metals into food chains: A 20-year comparison study in Northern Moravia (Czech Republic). Acta Veterinaria Brno. 2008;77:645–652. https://doi.org/10.2754/avb200877040645
  3. Dvornikov MG, Domskiy IA, Shiryaev VV, Sergeev AA. Ecology, conservation, and use of game resources in the North-East of Europe. Kirov: VESI; 2021. 291 p. (In Russ.).
  4. Kaledin AP, Stepanova MV. Bioaccumulation of trace elements in vegetables grown in various anthropogenic conditions. Foods and Raw Materials. 2023;11(1):10–16. https://doi.org/10.21603/2308-4057-2023-1-551
  5. Bat L, Arici E, Öztekin A, Şahin Fa. Farmed Turkish salmon: Toxic metals and health threat. Foods and Raw Materials. 2021;9(2):317–323. https://doi.org/10.21603/2308-4057-2021-2-317-323
  6. Bires J, Maracek I, Bartko P, Biresova M, Weissova T. Accumulation of trace elements in sheep and the effects upon qualitative and quantitative ovarian changes. Veterinary and Human Toxicology. 1995;37(4):349–356.
  7. Rous P, Jelinek P. The effect of increased soil contamination with heavy metals on their content in some rabbits tissues. Czech Journal of Animal Science. 2000;45(7):319–324.
  8. Toman R, Massány P. Cadmium in selected organs of fallow-deer (Dama dama), sheep (Ovis aries), Brown hare (Lepus europaeus) and Rabbit (Oryctolagus cuniculus) in Slovakia. Journal of Environmental Science and Health. Part A: Environmental Science and Engineering and Toxicology. 1996;31(5):1043–1051. https://doi.org/10.1080/10934529609376406
  9. Saveljev A, Sergeyev A, Grebnev I, Chirkov S, Sotnikov V. The hunting resources of a green belt of a big city in the East of Europe: number, sanitary parameters, and possibilities of sustainable harvest. Beiträge zur Jagd – und Wildforschung. 2013;38:69–80.
  10. Tataruch F. Roe deer antlers as biomonitors for lead contamination. Bulletin of Environmental Contamination and Toxicology. 1995;55:332–337. https://doi.org/10.1007/BF00206669
  11. Rattner BA, Shore RF. Ecotoxicology of wild mammals (Ecological and environmental toxicology series). Wiley; 2001. 752 p.
  12. Sergeyev A, Safonov V. Investigations on the ecotoxicology of game animals in Russia. Proceedings of XXVIIth Congress of the International Union of Game Biologists; 2005; Hannover. Hamburg: Verlag Hamburg; 2005. p. 470–471.
  13. Baikov B, Krynski A, Stoyanov M, Ivanova M, Guguva M, Topalski E, et al. Heavy metals in hare organs as bioindicators for environment pollution. Proceedings of the Bulgarian Academy of Sciences. 1994;47:93–96.
  14. Safonov VG, Yegoshina TL, Sergeyev AA, Saveljev AP, Skumatov DV, Orlov PP, et al. Methodological aspects and opportunities for practical use of bioindication of biological objects. Modern Problems of Bioindication and Biomonitoring: Proceedings of the XI International Symposium on Bioindicators; 2001; Syktyvkar. Syktyvkar: FRC Komi SC UB RAS; 2001. p. 367–375. (In Russ.).
  15. Pulliainen E, Lajunen LHJ, Itamies J, Anttila R. Lead and Cadmium in the liver and muscles of the mountain hare (Lepus timidus) in Northern Finland. Annales Zoologici Fennici. 1984;21(2):149–152.
  16. Kålås JA, Ringsby TH, Lierhagen S. Metals and selenium in wild animals from Norwegian areas close to Russian nickel smelters. Environmental Monitoring and Assessment. 1995;36:251–270. https://doi.org/10.1007/BF00547905
  17. Venäläinen E-R, Niemi A, Hirvi T. Heavy metals in tissues of hares in Finland, 1980–82 and 1992–93. Bulletin of Environmental Contamination and Toxicology. 1996;56:251–258. https://doi.org/10.1007/s001289900038
  18. Zarubin BE, Ekonomov AV, Kolesnikov VV, Shevnina MS, Sergeev AA. The resources of mountain hare in the Kirov region and their use. Far Eastern Agrarian Bulletin. 2021;60(4):87–102. (In Russ.). https://doi.org/10.24412/1999-6837-2021-4-87-102
  19. Ivanter EhV, Korosov AV. Biology of chemical elements. Petrozavodsk: PetrGU; 2010. 104 p. (In Russ.).
  20. Fidalgo L, de La Cruz B, Goicoa A, Espino L. Accumulation of zinc, copper, cadmium and lead in liver and kidney of the iberian hare (Lepus granatensis) from Spain. Research and Reviews. Journal of Veterinary Sciences. 2016;2(1):15–20.
  21. Potthast K. Residues in meat and meat products. Fleischwirtsch. 1993;73:432–434.
  22. Ahmed MS, Azam MA, Ahmed KS, Ali H. Accumulation of some heavy metals in selected tissues of cape hare, Lepus capensis from Pakistan. Pakistan Journal of Wildlife Archives. 2016;7(2):11–20.
  23. Pedersen S, Lierhagen S. Erratum to “Heavy metal accumulation in arctic hares (Lepus arcticus) in Nunavut, Canada”. Science of the Total Environment. 2006;370(2–3):588. https://doi.org/10.1016/j.scitotenv.2006.08.025
  24. Demirbaş Y, Erduran N. Concentration of selected heavy metals in brown hare (Lepus europaeus) and wild boar (Sus scrofa) from Central Turkey. Balkan Journal of Wildlife Research. 2017;4(2):26–33. https://doi.org/10.15679/bjwr.v4i2.54
  25. Wajdzik M. Contents of cadmium and lead in liver, kidneys and blood of the European hare (Lepus europaeus Pallas 1778) in Malopolska. Journal Acta Scientiarum Polonorum Silvarum Colendarum Ratio et Industria Lignaria. 2006;5(2):135–146.
  26. Halecki W, Gąsiorek M, Wajdzik M, Pająk M, Kulak D. Population parameters including breeding season of the european brown hare (Lepus europaeus) exposed to cadmium and lead pollution. Fresenius Environmental Bulletin. 2017;26(4):2998–3004.
  27. Massányi P, Tataruch F, Slameka J, Toman R, Jurík R. Accumulation of lead, cadmium, and mercury in liver and kidney of the brown hare (Lepus europaeus) in relation to the season, age, and sex in the West Slovakian Lowland. Journal of Environmental Science and Health, Part A. Toxic/Hazardous Substances and Environmental Engineering. 2003;38(7):1299–1309. https://doi.org/10.1081/ese-120021127
  28. Beuković D, Vukadinović M, Krstović S, Polovinski-Horvatović M, Jajić I, Popović Z, et al. The European hare (Lepus europaeus) as a biomonitor of lead (Pb) and cadmium (Cd) occurrence in the agro biotope of Vojvodina, Serbia. Animals. 2022;12(10). https://doi.org/10.3390/ani12101249
  29. Petrović Z, Teodorović V, Djurić S, Milićević D, Vranić D, Lukić M. Cadmium and mercury accumulation in European hare (Lepus europaeus): Age-dependent relationships in renal and hepatic tissue. Environmental Science and Pollution Research. 2014;21(24):14058–14068. https://doi.org/10.1007/s11356-014-3290-0
  30. Kramárová M, Massányi P, Slamecka J, Tataruch F, Jancová A, Gasparik J, et al. Distribution of cadmium and lead in liver and kidney of some wild animals in Slovakia. Journal of Environmental Science and Health. Part A. Toxic/Hazardous Substances and Environmental Engineering. 2005;40(3):593–600. https://doi.org/10.1081/ESE-200046605
  31. Oberlis D, Kharland B, Skalʹnyy A. Biology of macro- and microelements in humans and animals. St. Petersburg: Nauka; 2008. 542 p. (In Russ.).
  32. Timofeeva SN, Kadikov IR, Khaybullin RR. The effect of zinc on weight gain, biochemical parameters and the content of metals in organs of rats exposed to cadmium chloride. Vestnik of Mari State University. Chapter: Agriculture. Economics. 2020;6(1):59–66. (In Russ.). https://doi.org/10.30914/2411-9687-2020-6-1-59-65
  33. Potapova SN. The effect of magnesium and zinc on rats treated with cadmium. Veterinarian. 2021;(1):44–49. (In Russ.). https://doi.org/10.33632/1998-698X.2021-1-44–50
  34. Ma W. Effect of soil pollution with metallic lead pellets on lead bioaccumulation and organ/body weight alterations in small mammals. Archives of Environmental Contamination and Toxicology. 1989;18:617–622. https://doi.org/10.1007/BF01055030
  35. Sergeev AA, Shiryaev VV, Dvornikov MG, Tetera VA. Lead poisoning of wild animals and prospects for the use of non-toxic hunting ammunition in Russia. Far Eastern Agrarian Bulletin. 2020;53(1):71–83. (In Russ.). https://doi.org/10.24411/1999-6837-2020-11010
  36. Kottferová J, Korénedová B. Distribution of Cd and Pb in the tissues and organs of free-living animals in the territory of Slovakia. Bulletin of Environmental Contamination and Toxicology. 1998;60:171–176. https://doi.org/10.1007/s001289900607
  37. Ehykhler V. Poisons in our diet. Moscow: Mir; 1985. 214 p. (In Russ.).
  38. Alonso ML, Benedito JL, Miranda M, Castillo C, Hernández J, Shore RF. Arsenic, cadmium, lead, copper and zinc in cattle from Galicia. NW Spain. Science of the Total Environment. 2000;10:246(2–3):237–248. https://doi.org/10.1016/s0048-9697(99)00461-1
  39. Miranda M, Alonso ML, Castillo C, Hernández J, Benedito JL. Effect of sex on arsenic, cadmium, lead, copper and zinc accumulation in calves. Veterinary and Human Toxicology. 2000;42(5):265–268.
  40. Shore RF, Douben PET. The ecotoxicological significance of cadmium intake and residues in terrestrial small mammals. Ecotoxicology and Environmental Safety. 1994;29(1):101–112. https://doi.org/10.1016/0147-6513(94)90035-3
How to quote?
Kochkarev PV, Koshurnikova MA, Sergeyev AA, Shiryaev VV. Trace Elements in the Meat and Internal Organs of the Mountain Hare (Lepus timidus L., 1758) in the North of the Krasnoyarsk Region. Food Processing: Techniques and Technology. 2023;53(2):217–230. (In Russ.). https://doi.org/10.21603/2074-9414-2023-2-2436 
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