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Title of article PHYSICOCHEMICAL PROPERTIES AND BIOLOGICAL ACTIVITY OF EXTRACTS OF DRIED BIOMASS OF CALLUS AND SUSPENSION CELLS AND IN VITRO ROOT CULTURES
Authors

Yang Y., Professor of the College of Food and Bioengineering, Qiqihar University, yangyong7904@163.com

Asyakina L., Cand.Sci.(Eng.), Associate Professor of the Department of BioNano-technology, Kemerovo State University, alk_kem@mail.ru

Babich O., Dr.Sci.(Eng.), Director of the Institute of Living Systems, Immanuel Kant Baltic Federal University, olich.43@mail.ru

Dyshluk L., Dr.Sci.(Bio.), Associate Professor of the Department of BioNano-technology, Kemerovo State University, soldatovals1984@mail.ru

Sukhikh S., ICand.Sci.(Eng.), Head of the Laboratory, Immanuel Kant Baltic Federal University, stas-asp@mail.ru

Popov A., Research, Immanuel Kant Baltic Federal University, ADpopov@kantiana.ru

Kostyushina N., Associate Professor, Immanuel Kant Baltic Federal University, NiKostiushina@kantiana.ru

Section
Year 2020 Issue 3 UDC 664.833:602.4
DOI 10.21603/2074-9414-2020-3-480-492
Abstract Introduction. One of the urgent problems of medicine and biology is the use of plant objects as industrial producers of target metabolites in vitro. In vitro cells can be used as pharmaceutical preparations.
Study objects and methods. The present research featured medicinal plants that grow in the Siberian Federal district and are a popular source of medicinal raw materials. The physicochemical properties, e.g. total ash content in extracts, the content of heavy metals, the content of organic solvents in the extracts, and the mass loss upon drying was determined by standard methods. The antimicrobial properties of in vitro extracts were determined by the diffusion method and the method based on optical density measurement. The list of opportunistic and pathogenic test strains included the following microorganisms: E. coli ATCC 25922, S. aureus ATCC 25923, P. vulgaris ATCC 63, P. aeruginosa ATCC 9027, and C. albicans EMTC 34. The number of viable cancer cells was determined using the MTT colorimetric method.
Results and discussion. The paper describes the physicochemical properties, safety indicators, antioxidant activity, antimicrobial activity, and antitumor properties of extracts of a complex of biologically active substances obtained in vitro from the dried biomass of callus and suspension cell cultures and root cultures. The root extracts proved to have the maximum antimicrobial and cytotoxic properties. They could reduce the survival rate of cancer cells to 24.8–36.8 %.
Conclusion. The research featured extracts obtained from the dried biomass of callus and suspension cell cultures and root cultures in vitro of safflower leuzea (Leuzea carthamoides L.), Rhodiola rosea (Rhodiola rosea L.), various sorts of skullcap (Scutellaria baicalensis L., Scutellaria andrachnoides L., Scutellaria galericulata L.), Potentilla alba (Potentilla alba L.) and ginseng (Panax L.). The results showed that the extracts can be used for the production of pharmaceuticals and biologically active additives with antitumor, antimicrobial, and antioxidant properties.
Keywords Medicinal plants, biologically active additives, plant cells, cell culture, bacteria
Artice information Received June 29, 2020
Accepted August 28, 2020
Available online October 8, 2020
For citation Yang Y, Asyakina LK, Babich OO, Dyshlyuk LS, Sukhikh SA, Popov AD, et al. Physicochemical properties and biological activity of extracts of dried biomass of callus and suspension cells and in vitro root cultures. Food Processing: Techniques and Technology. 2020;50(3):480–492. (In Russ.). DOI: https://doi.org/10.21603/2074-9414-2020-3-480-492.
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