Rus / Eng

ISSN 2074-9414 (Print)

ISSN 2313-1748 (Online)
Publishing office, Editorial office:

Kemerovo State University

Alexander Prosekov

Executive Editor:
Anna Loseva

Publishing Editor:
Alena Kiryakova

Online Media Registration Number:
EL FS 77 - 72312 (01.02.2018)

6 Krasnaya Str.,
Kemerovo 650000,
tel.: +7 (3842) 58-80-24
Submit manuscript

Article information

Views: 112


Baurina A., D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia,

Baurin D., D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia

Shakir I., D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia

Panfilov V., D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia

Year 2021 Issue 1 UDC 663.911.15:60
DOI 10.21603/2074-9414-2021-1-134-145
Abstract Introduction. Obtaining protein and vitamin fodder is one of the urgent tasks that modern industrial biotechnology has to solve. Another task is a search of novel medium compositions for microbial fermentation that can lower production costs. Russian food industry produces a significant amount of sunflower seed processing byproducts every year. Sunflower meal is a promising source of sunflower protein isolate. The research objective was to develop a new technology for the production of Bacillus megaterium bacterial biomass for fodder purposes.
Study objects and methods. The research featured a sunflower protein isolate, an enzyme complex Protex 7L, and a B. megaterium strain (VKPM B-3750). The carbohydrate content was determined using a modified Bertrand method. Amine nitrogen was studied using formol titration, the number of viable cells – by the Koch method, the content of amino acids – by capillary electrophoresis.
Results and discussion. When processed with enzyme complexes, sunflower protein can be an alternative source of nitrogen for industrial fermentation. The study featured amino acid of sunflower protein isolate and enzymatic hydrolyzate obtained using Protex 7L. A comparative analysis of the content of amino acids in the hydrolyzate and the protein isolate showed that enzymatic hydrolysis can significantly increase the content of free amino acids in the medium available for microbial accumulation. The research proved that sunflower protein enzymatic hydrolyzate obtained using Protex 7L can be used to cultivate strains of B vitamins producers.
Conclusion. Sunflower protein enzymatic hydrolyzate can be used as a nitrogen source for B vitamins producer fermentation and as an alternative to expensive meat peptone. The research involved technical and economic assessment of the B. megaterium fermentation on enzymatic hydrolysates of sunflower protein at a production capacity of 100 kg per year. The cost of the protein-vitamin supplement was calculated as 413 rubles per kg, while the market price could reach 826 rubles per kg. The payback period for capital expenditures was estimated at 1.5 years. Thus, replacing commercial meat peptone with sunflower protein enzymatic hydrolyzate obtained with Protex 7L reduced the cost of 1 kg of feed additive by three times without affecting B. megaterium. Overproduction of B vitamins by the B. megaterium strain on a medium containing sunflower protein hydrolyzate requires optimization of fermentation conditions.
Keywords Sunflower, sunflower meal, protein isolate, fermentation, enzymatic hydrolysis, feed additive
Artice information Received December 10, 2020
Accepted February 7, 2021
Available online March 25, 2021
For citation Baurina AV, Baurin DV, Shakir IV, Panfilov VI. Technology for the Bacillus megaterium Fodder Biomass Production. Food Processing: Techniques and Technology. 2021;51(1):134–145. (In Russ.).
  1. Panesar PS, Kaur R, Singla G, Sangwan RS. Bio-processing of agro-industrial wastes for production of food-grade enzymes: Progress and prospects. Applied Food Biotechnology. 2016;3(4):208–227.
  2. Berrazaga I, Micard V, Gueugneau M, Walrand S. The role of the anabolic properties of plant-versus animal-based protein sources in supporting muscle mass maintenance: A critical review. Nutrients. 2019;11(8).
  3. Sabaté J, Sranacharoenpong K, Harwatt H, Wien M, Soret S. The environmental cost of protein food choices. Public Health Nutrition. 2015;18(11):2067–2073.
  4. Albe Slabi S, Mathe C, Basselin M, Framboisier X, Ndiaye M, Galet O, et al. Multi-objective optimization of solid/ liquid extraction of total sunflower proteins from cold press meal. Food Chemistry. 2020;317. foodchem.2020.126423.
  5. Domoroshchenkova ML, Demjanenko TF, Krilova IV, Kamisheva IM. Protein opportunities of sunflower seeds. Research of processes of food protein production from sunflower oil meal. Vestnik of the All-Russia Scientific Research Institute of Fats. 2020;(1–2):24–29. (In Russ.).
  6. Subaşı BG, Casanova F, Capanoglu E, Ajalloueian F, Sloth JJ, Mohammadifar MA. Protein extracts from de-oiled sunflower cake: Structural, physico-chemical and functional properties after removal of phenolics. Food Bioscience. 2020;38. https://
  7. Sara AS, Mathé C, Basselin M, Fournier F, Aymes A, Bianeis M, et al. Optimization of sunflower albumin extraction from oleaginous meal and characterization of their structure and properties. Food Hydrocolloids. 2020;99. foodhyd.2019.105335.
  8. Makarova AS, Baurin DV, Gordienko MG, Kudryavtseva EI. Green chemistry for the optimum technology of biological conversion of vegetable waste. Sustainable Production and Consumption. 2017;10:66–73.
  9. Alagawany M, Attia AI, Ibrahim ZA, Mahmoud RA, El-Sayed SA. The effectiveness of dietary sunflower meal and exogenous enzyme on growth, digestive enzymes, carcass traits, and blood chemistry of broilers. Environmental Science and Pollution Research. 2017;24(13):12319–12327.
  10. Gaviley EV, Panʹkova SN, Katerinich OA. Vliyanie chastichnoy zameny soevogo shrota polsolnechnym kontsentratom v ratsione tsyplyat-broylerov na produktivnostʹ i fiziologicheskoe sostoyanie ptitsy [Influence of partial replacement of soybean meal with sunflower concentrate in broiler chicken ration on productivity and physiological state of poultry]. Aktualʹnye problemy intensivnogo razvitiya zhivotnovodstva [Actual problems of intensive development animal husbandry]. 2020;(23–1):120–127. (In Russ.).
  11. Mustafayev SK, Smychagin EO. Organization of fodder production based on sunflower seed waste. Advances in Engineering Research. 2018;157:429–434.
  12. Fang H, Kang J, Zhang D. Microbial production of vitamin B12: a review and future perspectives. Microbial Cell Factories. 2017;16(1).
  13. Wang P, Zhang Z, Jiao Y, Liu S, Wang Y. Improved propionic acid and 5,6-dimethylbenzimidazole control strategy for vitamin B12 fermentation by Propionibacterium freudenreichii. Journal of Biotechnology. 2015;193:123–129. https://doi. org/10.1016/j.jbiotec.2014.11.019.
  14. Hajfarajollah H, Mokhtarani B, Mortaheb H, Afaghi A. Vitamin B12 biosynthesis over waste frying sunflower oil as a cost effective and renewable substrate. Journal of Food Science and Technology. 2015;52(6):3273–3282. 014-1383-x.
  15. Li K-T, Peng W-F, Zhou J, Wei S-J, Cheng X. Establishment of beet molasses as the fermentation substrate for industrial vitamin B12 production by Pseudomonas denitrificans. Journal of Chemical Technology and Biotechnology. 2013;88(9):1730–1735.
  16. Acevedo-Rocha CG, Gronenberg LS, Mack M, Commichau FM, Genee HJ. Microbial cell factories for the sustainable manufacturing of B vitamins. Current Opinion in Biotechnology. 2019;56:18–29.
  17. Patelski P, Berlowska J, Balcerek M, Dziekonska-Kubczak U, Pielech-Przybylska K, Dygas D, et al. Conversion of potato industry waste into fodder yeast biomass. Processes. 2020;8(4).
  18. Baurin DV, Epishkina JM, Baurina AV, Shakir IV, Panfilov VI. Sunflower protein enzymatic hydrolysates as a medium for vitamin B2 and B12 biosynthesis. Chemical Engineering Transactions. 2020;79:145–150.
  19. Dabbour M, He R, Mintah B, Golly MK, Ma H. Ultrasound pretreatment of sunflower protein: Impact on enzymolysis, ACE-inhibition activity, and structure characterization. Journal of Food Processing and Preservation. 2020;44(4). https://doi. org/10.1111/jfpp.14398.
  20. Dabbour M, Xiang J, Mintah B, He R, Jiang H, Ma H. Localized enzymolysis and sonochemically modified sunflower protein: Physical, functional and structure attributes. Ultrasonics Sonochemistry. 2020;63. ultsonch.2019.104957.