ISSN 2074-9414 (Print),
ISSN 2313-1748 (Online)

Effect of Light on Rhizogenesis of Forest Berry Plants during Clonal Micropropagation

Abstract
Introduction. Forest berry plants are popular on the food market and in pharmacy for their high nutritional and medicinal value. Plantations of forest berry plants can proliferate on unused lands, including depleted peatlands. Clonal micropropagation is the most effective method for obtaining large quantities of high quality planting material. Light-emitting diodes are highly effective for clonal micropropagation. The research objective was to study the effect of different spectral ranges on the process of root formation of forest berry plants in vitro. Study objects and methods. The research featured regenerant plants of half-highbush blueberry, arctic bramble, American cranberry, European cranberry, lingonberry, and Kamchatka bilberry of different cultivars. A set of experiments made it possible to study the effect of lighting type on the growth and development of the root system of forest berry plants in vitro using white fluorescent lamps, white spectrum LED lamps, and LED lamps with a combination of white, red, and blue spectra at the in vitro rooting stage of clonal micropropagation. Results and its discussion. The largest number (3.4–14.6 pcs.) and the maximum total length (10.0–156.9 cm) of roots were observed under LED lamps with a combination of white, red, and blue spectra. The effect was by 1.1–2.8 and 2.0–4.5 times higher than in the case of white-spectrum LED lamps, and by 2.3–7.0 and 3.3–14.9 times than in the case of fluorescent lamps. Variety and shape proved to have no significant effect on biometric indicators. Conclusion. LED lamps had a positive effect on the process of rhizogenesis of forest berry plants during clonal micropropagation. They appeared to be more effective than fluorescent lamps. The combination of white, blue, and red spectra increased the biometric parameters of plants at the stage of in vitro rooting.
Keywords
Clonal micropropagation, in vitro, root formation blueberry, arctic bramble, cranberry, lingonberry, Kamchatka bilberry, LED lamps, range
REFERENCES
  1. Tyak GV. Vyrashchivaem knyazheniku [Arctic bramble and how to grow it]. Pitomnik i chastnyy sad [Nursery and Garden]. 2016;(1):18–22. (In Russ.).
  2. Dróżdż P, Šėžienė V, Pyrzynska K. Phytochemical properties and antioxidant activities of extracts from wild blueberries and lingonberries. Plant Foods for Human Nutrition. 2017;72(4):360–364. https://doi.org/10.1007/s11130-017-0640-3.
  3. Fu Z, Liska D, Talan D, Chung M. Cranberry reduces the risk of urinary tract infection recurrence in otherwise healthy women: A systematic review and meta-analysis. Journal of Nutrition. 2017;147(12):2282–2288. https://doi.org/10.3945/jn.117.254961.
  4. Li D, Li B, Ma Y, Sun X, Lin Y, Meng X. Polyphenols, anthocyanins, and flavonoids contents and the antioxidant capacity of various cultivars of highbush and half-high blueberries. Journal of Food Composition and Analysis. 2017;62:84–93. https://doi.org/10.1016/j.jfca.2017.03.006.
  5. Peron G, Sut S, Pellizzaro A, Brun P, Voinovich D, Castagliuolo I, et al. The antiadhesive activity of cranberry phytocomplex studied by metabolomics: Intestinal PAC-A metabolites but not intact PAC-A are identified as markers in active urines against uropathogenic Escherichia coli. Fitoterapia. 2017;122:67–75. https://doi.org/10.1016/j.fitote.2017.08.014.
  6. Ragnar M. Åkerbär. Black Island Books; 2017. 176 p.
  7. Angelova SG, Ivanova SKr, Trifonova I, Voleva S, Georgieva I, Stoyanova A, et al. Vaccinium vitis-idaea L., origin from Bulgaria indicate in vitro antitumor effect on human cervical and breast cancer cells. American Scientific Research Journal for Engineering, Technology, and Sciences. 2019;56(1):104–112.
  8. Debnath SC, An D. Antioxidant properties and structured biodiversity in a diverse set of wild cranberry clones. Heliyon. 2019;5(4). https://doi.org/10.1016/j.heliyon.2019.e01493.
  9. Philip N, Walsh LJ. Cranberry polyphenols: Natural weapons against dental caries. Dentistry Journal. 2019;7(1). https://doi.org/10.3390/dj7010020.
  10. Rocha DMUP, Caldas APS, da Silva BP, Hermsdorff HHM, Alfenas RDCG. Effects of blueberry and cranberry consumption on type 2 diabetes glycemic control: A systematic review. Critical Reviews in Food Science and Nutrition. 2019;59(11):1816–1828. https://doi.org/10.1080/10408398.2018.1430019.
  11. Coleman CM, Ferreira D. Oligosaccharides and complex carbohydrates: A new paradigm for cranberry bioactivity. Molecules. 2020;25(4). https://doi.org/10.3390/molecules25040881.
  12. Kalt W, Cassidy A, Howard LR, Krikorian R, Stull AJ, Tremblay F, et al. Recent research on the health benefits of blueberries and their anthocyanins. Advances in Nutrition. 2020;11(2):224–236. https://doi.org/10.1093/advances/nmz065.
  13. Tyak GV, Kurlovich LE, Tyak AV. Biological recultivation of degraded peatlands by creating forest berry plants. Vestnik of the Kazan State Agrarian University. 2016;11(2):43–46. (In Russ.). https://doi.org/10.12737/20633.
  14. Matsneva OV, Tashmatova LV. Clonal micro-propagation of strawberries is a promising method of modern nursery practice (review). Contemporary Horticulture. 2019;(4):113–119. (In Russ.). https://doi.org/10.24411/2312-6701-2019-10411.
  15. Makarov SS, Kuznetsova IB, Smirnov VS. Improving technology of clonal micropropagation of arctic bramble (Rubus arcticus L.). Forestry Information. 2018;4:91–97. (In Russ.). https://doi.org//10.24419/LHI.2304-3083.2018.4.09.
  16. Korenev IA, Tyak GV, Makarov SS. Creation of new varieties of forest berry plants and prospects of their intensive reproduction (in vitro). Forestry Information. 2019;3:180–189. (In Russ.). https://doi.org/10.24419/LHI.2304-3083.2019.3.15.
  17. Makarov SS, Kuznetsova IB, Upadyshev MT, Rodin SA, Chudetsky AI. Clonal micropropagation of cranberry (Oxycoccus palustris Pers.). Food Processing: Techniques and Technology. 2021;51(1):67–76. (In Russ.). https://doi.org/10.21603/2074-9414-2021-1-67-76.
  18. Makarov SS, Kuznetsova IB, Chudetsky AI, Rodin SA. Obtaining high-quality planting material of forest berry plants by clonal micropropagation for restoration of cutover peatlands. Lesnoy zhurnal. 2021;380(2):21–29.
  19. Tikhomirov AA, Ushakova SA. Nauchnye i tekhnologicheskie osnovy formirovaniya fototrofnogo zvena biologo-tekhnicheskikh sistem zhizneobespecheniya [Scientific and technological foundations of the phototrophic link of biological and technical life support systems]. Krasnoyarsk: Reshetnev Siberian State University of Science and Technology; 2016. 200 p. (In Russ.).
  20. Hung CD, Hong C-H, Kim S-K, Lee K-H, Park J-Y, Nam M-W, et al. LED light for in vitro and ex vitro efficient growth of economically important highbush blueberry (Vaccinium corymbosum L.). Acta Physiologiae Plantarum. 2016;38(6). https://doi.org/10.1007/s11738-016-2164-0.
  21. Jung W-S, Chung I-M, Hwang MH, Kim S-H, Yu CY, Ghimire BK. Application of light-emitting diodes for improving the nutritional quality and bioactive compound levels of some crops and medicinal plants. Molecules. 2021;26(5). https://doi.org/10.3390/molecules26051477.
How to quote?
Makarov SS, Rodin SA, Kuznetsova IB, Chudetsky AI, Tsaregradskaya SYu. Effect of Light on Rhizogenesis of Forest Berry Plants during Clonal Micropropagation. Food Processing: Techniques and Technology. 2021;51(3):520–528. (In Russ.). https://doi.org/10.21603/2074-9414-2021-3-520-528.
About journal

Download
Contents
Abstract
Keywords
References