Abstract

The present research features a new biotechnological approach for complex processing of secondary cereal products. The approach makes it possible to obtain new functional foods and ingredients, such as carbohydrate-protein concentrate, dietary fiber concentrates, biologically active substances, polyphenols, and xylo-oligosaccharides. The complex biotechnology involves oat bran biotransformation and includes chemical, hydrothermal, and enzymatic methods of extraction, which allows manufacturers to obtain functional ingredients with antioxidant and prebiotic properties. The concentrates obtained as a result of the study contained a significant amount of protein, hydrolysis products of polysaccharides (glucose, maltodextrins), and free polyphenols. The experiment produced concentrates of biologically active substances containing xylo-oligosaccharides and phenolic compounds. The content of polyphenols was 67% of the total amount in oat bran, protein – 6.9%, carbohydrates – 80.7% xylo-oligosaccharides whith prebiotic properties 35.3%–71.5%, and ash 11.3%. The obtained data indicated a high antioxidant activity of polyphenol extracts. The differences in antioxidant activity between various methods of extraction are associated primarily with the completeness of extraction and the stability of the extracted phenolic compounds. Ultrasonic treatment proved beneficial for the extraction kinetics and the polyphenol yield at the initial stage, with an increase in antioxidant properties. As for the antioxidant activity of polyphenol concentrate during storage, the research did not reveal changes for 8 months at a temperature of 20 ± 1°C and a relative humidity of 70 ± 5%. The study of the growth dynamics of Lactobacillus acidophilus and Bifi obacterium bifi um in media containing xylo-oligosaccharide concentrate confirmed the presence of prebiotic properties and their selectivity. The accumulation of biomass of prebiotic cultures occurred faster with the use of nutrient media with the use of xylo-oligosaccharide concentrate and lactulose, as compared with milk. Thus, the research confirmed the feasibility of biotechnology for transforming oat bran into functional ingredients, which makes it possible to use them in new technological solutions for products with bifidogenic properties.

Keywords

Phytosubstance, prebiotics, xylooligosaccharides, secondary products of grain processing

REFERENCES

1. Chlopicka J, Pasko P, Gorinstein S, Jedryas A, Zagrodzki P. Total phenolic and total flavonoid content, antioxidant activity and sensory evaluation of pseudocereal breads. LWT – Food Science and Technology. 2012;46(2):548–555. DOI: https://doi. org/10.1016/j.lwt.2011.11.009.
2. Dwivedia S, Sahrawat K, Puppala N, Ortiz R. Plant prebiotics and human health: Biotechnology to breed prebiotic-rich nutritious food crops. Electronic Journal of Biotechnology. 2014;17(5):238–245. DOI: https://doi.org/10.1016/j.ejbt.2014.07.004.
3. Gibson GR, Rastall RA. Prebiotics: Development and Application. England: John Wiley and Sons Ltd; 2006. 256 p. DOI: https://doi.org/10.1002/9780470023150.
4. Gorbunova N, Bannikova A, Evteev A, Evdokimov I, Kasapis S. Alginate-based encapsulation of extracts from beta Vulgaris cv. beet greens: Stability and controlled release under simulated gastrointestinal conditions. Lwt-Food Science and Technology. 2018;93:442–449. DOI: https://doi.org/10.1016/j.lwt.2018.03.075.
5. Hole AS, Grimmer S, Jensen MR, Sahistrom S. Synergistic and suppressive effects of dietary phenolic acids and other phytochemicals from cereal extracts on nuclear factor kappa B activity. Food Chemistry. 2012;133(3):969–977. DOI: https://doi. org/10.1016/j.foodchem.2012.02.017.
6. Kaprelyants L, Zhurlova O. Biotechnological approaches for the production of functional foods and supplements from cereal raw materials. Food Science and Technology. 2014;27(2):15–19.
7. Kaprelyants L, Zhurlova O. Technology of wheat and rye bran biotransformation into functional ingredients. International Food Research Journal. 2017;24(5):1975–1979.
8. Kaprelyants LV, Voloshenko OS, Zhurlova ED. Bioaktivnye soedineniya i pishchevye volokna v novykh razrabotannykh zernovykh produktakh [Bioactive Compounds and Dietary Fibre in Advanced Cereal Products]. Grain Products and Mixed Fodder’s. 2012;47(3):17–21. (In Ukr.).
9. Kaur R, Ahluwalia P, Sachdev PA, Kaur A. Development of gluten-free cereal bar for gluten intolerant population by using quinoa as major ingredient. Journal of Food Science and Technology-Mysore. 2018;55(9):3584–3591. DOI: https://doi.org/10.1007/ s13197-018-3284-x.
10. Perales-Sánchez JXK, Reyes-Moreno C, Gomez-Favela MA, Milan-Carrillo J, Cuevas-Rodriguez E, Valdez-Ortiz A, et al. Increasing the Antioxidant Activity, Total Phenolic and Flavonoid Contents by Optimizing the Germination Conditions of Amaranth Seeds. Plant Foods for Human Nutrition. 2014;69(3):196–202. DOI: https://doi.org/10.1007/s11130-014-0430-0.
11. Reddy SS, Krishnan C. Production of prebiotics and antioxidants as health food supplements from lignocellulosic materials using multienzymatic hydrolysis. International Journal of Chemical Sciences. 2010;3(3)535–549.
12. Roberfroid MB. Prebiotics and probiotics: are they functional foods? American Journal of Clinical Nutrition. 2000;71(6):1682S–1687S. DOI: https://doi.org/10.1093/ajcn/71.6.1682S.
13. Singh RD, Banerjee J, Arora A. Prebiotic potential of oligosaccharides: A focus on xylan derived oligo-saccharides. Bioactive Carbohydrates and Dietary Fibre. 2015;5(1):19–30. DOI: https://doi.org/10.1016/j.bcdf.2014.11.003.
14. Tungland BC, Meuer D. Nondigestible Oligo – and Polysaccharides (Dietary Fiber): Their Physiology and Role in Human Health and Food. Comprehensive Reviews in Food Science and Food Safety. 2002;1(3):73–92. DOI: https://doi. org/10.1111/j.1541-4337.2002.tb00009.x.
15. Waters DM, Mauch A, Coffey A, Arendt EK, Zannini E. Lactic Acid Bacteria as a Cell Factory for the Delivery of Functional Biomolecules and Ingredients in Cereal-Based Beverages: A Review. Critical Reviews in Food Science and Nutrition. 2015;55(4):503–520. DOI: https://doi.org/10.1080/10408398.2012.660251.
16. State Standard 10444.11-2013. Microbiology of food and animal feeding stuffs. Methods for detection and enumeration of mesophilic lactic acid bacteria. Moscow: Standartinform; 2014.
17. State Standard 10444.11-89. Food products. Methods for determination of the lactic acid bacteria. Moscow: Standartinform; 2010.
18. MUK 4.2.999-00. Opredelenie kolichestva bifidobakteriy v kislomolochnykh produktakh [Recommended Practices No.4.2.999-00 Mesuring the amount of bifidobacteria in fermented milk products].
19. TR TS 021/2011. Tekhnicheskiy reglament Tamozhennogo soyuza “O bezopasnosti pishchevoy produktsii” [RP of the Customs Union 021/2011. Technical regulations of the Customs Union “On food safety”]. 2011.
20. Tutelyan VA, Lashneva NV. Biologically active substances of plant origin. Flavonols and flavones: prevalence, dietary sourses and consumption. Problems of Nutrition. 2013;82(1):4–22. (In Russ.).