Abstract
Examination of biological material samples were conducted to detect the effectiveness of the DNA isolation and purification quality. The optimum high performance method for isolating animal DNA from samples of biological origin and multicomponent compositions on its basis was selected. The quantitative content of animal DNA in samples of biological origin and multicomponent formulations on their basis was studied.Keywords
Protein, identification, diagnosis, infection, prion diseases of humans and animals, molecular and biological studies.REFERENCES
- Pokrovskiy, V.I. Priony i prionnye bolezni / V.I. Pokrovskiy, O.I. Kiselev, B.L. Cherkasskiy. – M.: RAMN, 2004. – 384 s.
- Internet-istochnik http://medicalplanet.su/genetica/201.html
- Somerville, R.A. Immunodetection of PrPSc in spleens of some scrapie infected sheep but not BSE infected cows / R.A. Somerville, C.R. Birkett, C.F. Farquhar // J. Gen. Virol. – 1997. – Vol. 78. – P. 2389–2396.
- Internet-istochnik http://www.eurolab.ua/encyclopedia/323/2260/
- Galkin, A.P. Priony drozhzhey, amiloidozy mlekopitayuschih i problema proteomnyh setey / A.P. Galkin, L.N. Mironova, G.A. Zhuravleva, S.G. Inge-Vechtomov // Genetika. – 2006. – T. 42. – № 11. – S. 1–13.
- Grigor'ev, V.B. Metody diagnostiki prionnyh zabolevaniy / V.B. Grigor'ev, A.N. Pokidyshev, S.L. Kal'nov, S.M. Klimenko // Voprosy virusologii. – 2009. – № 5. – S. 4–9.
- Grigor'ev, V.B. Prionnye bolezni cheloveka i zhivotnyh / V.B. Grigor'ev // Voprosy virusologii. – 2004. – T. 6. – S. 4–12.
- Li, R. Identification of an epitope in the C terminus of normal prion protein whose expression is modulated by binding events in the N terminus / R. Li, T. Liu, B.S. Wong et al. // J. Mol. Biol. – 2000. – Vol. 301. – P. 567–574.
- Apetri, A.C. Early Intermediate in Human Prion Protein Folding As Evidenced by Ultrarapid Mixing Experiments / A.C. Apetri, K. Maki, H. Roder, W.K. Surewicz // J. Am. Chem. Soc. – 2006. – Vol. 128. – P. 11673–11678.