Affiliation
a North Caucasus State Technical University
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Abstract
At present lactose and its derivatives are widely used in medicine, food industry, luxury cosmetics. Due to its unique properties, the lactobionic acid, a product of lactose oxidation is of special interest. Therefore, the development of a quick and reliable method for assessing the quality of hydroxy acid, and the products obtained during its synthesis is highly topical. One of the perspective directions for modern analysis of carbohydrates is chromatography but the method of high-performance liquid chromatography (HPLC) for lactobionic acid appeared to be labor-consuming and there are no data on gas-liquid chromatography (GLC). The possibility of using the gas-liquid chromatography to analyze the quality of lactobionic acid is shown; the method and optimal conditions of chromatography are described. It has been found that the best resolution of the peaks is reached in the mode of temperature programming from 175 to 260 °C at a rate of 6 deg/min and the sensitivity of flame ionization detector - 2∙10A. The time of lactobionic acid retention has been defined. It has been shown that it is not affected by the presence of lactose.
Keywords
Lactose,
lactobionic acid,
gas-liquid chromatography
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