Конструирование родоспецифичных и видоспецифичных праймеров для индикации и идентификации Lactobacillus bulgaricus

DESIGN OF GENUS-SPECIFIC AND SPECIES-SPECIFIC PRIMERS FOR INDICATION AND IDENTIFICATION OF LACTOBACILLUS BULGARICUS

Olga O. Babich ^a

E. V. Korotkaya ^a

Alexander Yu. Prosekov ^a

Affiliation

a ГОУ ВПО «Кемеровский технологический институт пищевой промышленности»

Copyright ©Bespomestnykh et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0. (http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.

Abstract

A comparative analysis of the nucleotide sequences of Lactobacillus deposited into the NCBI database has been carried out. Genus-specific and species-specific primers for indication and identification of L. bulgaricus and L. acidophilus have been synthesized. Genus-specific and species-specific primers designed have a low degree of homology regarding DNA regions of other bacteria of lactic fermentation that ensures their specificity. The developed method of indication of lactic acid bacteria and a complex scheme of their generical and specific identification can be used to characterize the isolated strains of Lactobacillus. It can be of practical interest for use in a system of quality control of foodstuffs enriched with Lactobacillus.

Keywords

Lactobacillus, Bacteria, genus, strain, phylogenetic analysis, primers, polymerase chain reaction (PCR)

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