ISSN 2074-9414 (Print),
ISSN 2313-1748 (Online)

Russian and Foreign Cultivars of Honeysuckle (Lonicera edulis Turcz.): cultivation studies in vitro

Introduction. The demand for honeysuckle berries and planting material is growing. Clonal micropropagation is the most effective method for industrial plantations. The research objective was to study the effect of cytokinins and auxins on Russian and Canadian honeysuckle microshoots and roots.
Study objects and methods. The study featured regenerated honeysuckle (Lonicera edulis Turcz.) of three Russian cultivars (Bakcharsky Velikan, Doch Velikana, Yugana) and two Canadian cultivars (Boreal Beauty, Boreal Beast). The experiment focused on the effect of sterilizing agents and sterilization time on the viability of honeysuckle explants at the stage of culture introduction in vitro. The effect of the growth regulator Cytodef in the QL nutrient medium on organogenesis was studied at the stage of micropropagation proper, the effect of auxin IBA on plant root formation – at the stage of rooting in vitro.
Results and discussion. The greatest viability of honeysuckle explants (80–94%) was registered in the samples affected by Lizoformin 3000 (5%) and silver nitrate (0.2%) as sterilizing agents with a sterilization time of 10 min at the stage of in vitro culture introduction. The biggest quantity (8.8 pcs.) and total length (40.1 cm) of microshoots were observed when the content of cytokinin Cytodef in the culture medium QL was 0.3 mg/L at the stage micropropagation proper. The Boreal Beast cultivar had the largest total length of shoots (29.0 cm). The biggest quantity (5.5 pcs.) and total length (30.8 cm) of roots resulted from 0.5 mg/L of auxin IBA at the stage of rooting in vitro. Coconut substrate produced the highest survival rate (92–99%) at the stage of adaptation to non-sterile conditions in vivo, with the greatest number of leaves (8.1–10.2 pcs.) observed in Canadian cultivars.
Conclusion. Cytodef and IBA proved to be effective growth-regulating substances for microplants of Russian and Canadian honeysuckle cultivars in vitro, which makes them promising for berry plantations.
Clonal micropropagation, in vitro, Lonicera, cultivar, growth regulators, rooting
  1. Kuklina AG, Sorokopudov VN, Upadyschev MT, Sorokopudova OA, Prischepina GA. Current state and trends of selection of the sweet-berry honeysuckle. Vestnik of the Russian Agricultural Science. 2017;(5):41–45. (In Russ.).
  2. Boyarskikh IG. Features of Lonicera caerulea L. reproductive biology. Agricultural Biology. 2017;52(1):200–210. (In Russ.).
  3. Sorokopudov VN, Kuklina AG, Upadyshev MT. Sorta sʺedobnoy zhimolosti: biologiya i osnovy kulʹtivirovaniya [Honeysuckle cultivars: biology and cultivation basics]. Moscow: All-Russian Institute of Selection and Technology of Horticulture and Nursery; 2018. 160 p. (In Russ.).
  4. Kucharska AZ, Fecka I. Identification of iridoids in edible honeysuckle berries (Lonicera caerulea L. var. kamtschatica Sevast.) by UPLC-ESI-qTOF-MS/MS. Molecules. 2016;21(9).
  5. Kucharska AZ, Sokól-Lȩtowska A, Oszmiánski J, Piórecki N, Fecka I. Iridoids, phenolic compounds and antioxidant activity of edible honeysuckle berries (Lonicera caerulea var. kamtschatica Sevast.). Molecules. 2017;22(3).
  6. Boyarskikh IG, Vasiliev VG, Kukushkina TA. The content of biologically active polyphenols Lonicera caerulea subsp. pallasii in natural conditions and the introduction. Chemistry of Plant Raw Material. 2018;(2):86–96. (In Russ.).
  7. Perova IB, Rylina EV, Eller KI, Akimov MYu. The study of the polyphenolic complex and iridoid glycosides in various cultivars of edible honeysuckle fruits Lonicera edulis Turcz. ex Freyn. Problems of Nutrition. 2019;88(6):88–99. (In Russ.).
  8. Korobkova TS, Sabaraikina SM. Antioxidant activity of the berries of Lonicera L. under the conditions of Central Yakutia. Arctic and Subarctic Natural Resources. 2020;25(4):92–99. (In Russ.).
  9. Ďurišová Ľ, Juríková T, Eliáš PJr, Mlček J. Reproductive biology of two edible honeysuckles [Lonicera edulis Turcz. ex Freyn., Lonicera kamtchatica (Sevast.) Pojark.] in the conditions of Southwestern Slovakia. Acta Scientiarum Polonorum, Hortorum Cultus. 2020;19(3):63–72.
  10. Obraztsova PA, Shmeleva IYu, Rozhnov ED. Perspektivy ispolʹzovaniya plodov zhimolosti v proizvodstve vinnykh napitkov [Prospects for the use of honeysuckle berries in wine production]. Lomonosovskie chteniya na Altae: Fundamentalʹnye problemy nauki i obrazovaniya: sbornik nauchnykh statey mezhdunarodnoy konferentsii [Lomonosov readings in Altai: Fundamental Problems of Science and Education: proceedings of the international conference]; 2017; Barnaul. Barnaul: Altay State University; 2017. p. 1054–1055. (In Russ.).
  11. Soboleva EV, Sergacheva ES, Smertina ES, Fedyanina LN, Lyakh VA, Gladyshchuk OS. The use of honeysuckle (Lonicera edulis) extract in baking technology. Journal of International Academy of Refrigeration. 2018;(1):26–32. (In Russ.).
  12. Yakimlyuk TA, Kotov LA. The application fatality in the various branches of the national economy. Molodezhʹ i nauka [Youth and Science]. 2018;(4). (In Russ.).
  13. Momot TV, Kushnerova NF. Justification of the choice of raw sources from far east flora for receiving the pharmaceutical preparations. Izvestia of Samara Scientific Center of the Russian Academy of Sciences. 2016;18(2):146–149. (In Russ.).
  14. Rutts AV. Improvement of breeding technology for edible honeysuckle. Subtropical and Ornamental Horticulture. 2018;(64):132–136. (In Russ.).
  15. Suchkova SA, Abzaltdenov TZ. Features of blue honeysuckle propagation by hardwood cuttings in the Tomsk region. Contemporary Horticulture. 2019;(2):105–110. (In Russ.).
  16. Matsneva OV, Tashmatova LV. Clonal micro-propagation of strawberries is a promising method of modern nursery practice (review). Contemporary Horticulture. 2019;(4):113–119. (In Russ.).
  17. Zapolsky YaS, Medvedeva TV, Natalchuk TA, Bublyk MO. Propagation of edible honeysuckle (Lonicera edulis Turcz) in in vitro conditions. Agricultural Science and Practice. 2018;5(2):18–26.
  18. Kutas E, Veyevnik A, Titok V, Ogorodnyk L. Morphogenesis of introduced varieties of Lonicera edulis Turcz. ex Freyn depending on composition of nutrient media. International Journal of Advanced Research in Biological Sciences. 2019;6(4):35–41.
  19. Kolbanova EV. Influence of fitohormones in the nutrient medium on the proliferation at the microplants of blue honeysuckle cultivars (Lonicera caerulea L. var. kamtschatica). Proceedings of the National Academy of Sciences of Belarus. Biological Series. 2020;65(1):88–97. (In Russ.).
  20. Markova MG, Somova EN. Improvement of clonal micropropagation of berry crops. Vestnik of the Kazan State Agrarian University. 2021;16(1):39–44. (In Russ.).
  21. Yang Y, Asyakina LK, Babich OO, Dyshlyuk LS, Sukhikh SA, Popov AD, et al. Physicochemical properties and biological activity of extracts of dried biomass of callus and suspension cells and in vitro root cultures. Food Processing: Techniques and Technology. 2020;50(3):480–492. (In Russ.).
  22. Makarov SS, Kuznetsova IB. Influence of growth regulators on organogenesis of honeyberry when clonic micropropagation. Vestnik NGAU. 2018;49(4):36–42. (In Russ.).
  23. Makarov SS, Kalashnikova EA, Rumyantseva EP. Productivity of edible honeysuckle depending on the technology of propagation. Vesting of Volga State University of Technology. Series: Forest. Ecology. Nature Management. 2018;39(3):76–83. (In Russ.).
  24. Korenev IA, Tyak GV, Makarov SS. Creation of new varietiesof forest berry plantsand prospects of their intensive reproduction (in vitro). Forestry Information. 2019;(3):180–189. (In Russ.).
How to quote?
Kulikova EI, Makarov SS, Kuznetsova IB, Chudetsky AI. Russian and Foreign Cultivars of Honeysuckle (Lonicera edulis Turcz.): cultivation studies in vitro. Food Processing: Techniques and Technology. 2021;51(4):712–722. (In Russ.).
About journal