ISSN 2074-9414 (Print),
ISSN 2313-1748 (Online)

Bioethanol Production from Miscanthus sinensis Cellulose by Bioconversion

Introduction. Cellulose-containing parts of herbs are an excellent source of alternative energy and can be used to produce biological ethanol. The present research aims at improving this fundamental and promising area of biotechnology. It introduces a new consortium of microorganisms that can saccharify while fermenting the substrate. Study objects and methods. The research featured technical cellulose obtained from Miscanthus sinensis using hydrotropic delignification and oxidation with pertrifluoroacetic acid. The ethanol content in the culture liquid was determined using an Agilent 7890B gas chromatograph with a flame ionization detector. The biocompatibility of the strains was studied by growing a direct co-culture in a dense nutrient medium. Results and discussion. The research objective was to create a new microbial consortium for the single-step production of bioethanol from Miscanthus sinensis cellulose. A set of biocompatibility experiments and cultivation conditions made it possible to select the optimal producers. The two developed microbial consortia required optimal compositions of culture media, which were determined by varying the ratio of components and measuring the yield of ethanol in the resulting culture liquid. Conclusion. The best consortium for Miscanthus sinensis cellulose consisted of Pichia stipites Y7124, Candida shehatae NCL3501, Kluyveromyces marxianus Y-4290, and Zymomonas mobilis 113 at a ratio of 1:1:1:1. The optimal parameters of bioethanol production included: temperature = 35 ± 1°C, pH = 5.2, time = 16 ± 1 h. The most efficient culture medium had the following composition (g/l): glucose – 5.0; peptone – 5.0; yeast extract – 0.4; K2HPO4 – 1.5; (NH)2 HPO4 – 1.5; MgSO4 – 0.5.
Bioethanol, bioconversion, consortium, Miscanthus, technical cellulose, single-stage fermentation
  1. Makarova EI, Budaeva VV. Bioconversion of non-food cellulosic biomass. Part 1. Proceedings of Universities. Applied Chemistry and Biotechnology. 2016;6(2)(17):43–50. (In Russ.). https:/
  2. Tsapko YuL, Kholodnaya AS. Changes of biological activity of degraded chernozems in Kharkiv region due to giant miscanthus cultivation. Colloquium-journal. 2017;10(10):9–11. (In Russ.).
  3. Skiba EA, Gladysheva EK, Golubev DS, Budaeva VV, Aleshina LA, Sakovich GV. Self-standardization of quality of bacterial cellulose produced by Medusomyces gisevii in nutrient media derived from Miscanthus biomass. Carbohydrate Polymers. 2021;252.
  4. Redcay S, Koirala A, Liu J. Effects of roll and flail conditioning systems on mowing and baling of Miscanthus × giganteus feedstock. Biosystems Engineering. 2018;172:134–143.
  5. Gismatulina YuA. Comparison of quality of paper specimens derived by the combined method from miscanthus leaf and stem. Fundamental research. 2016;(8–2):243–247. (In Russ.).
  6. Gismatulina YuA, Sevastyanova YuV, Budaeva VV, Zolotukhin VN. Structural-dimensional characteristics of miscanthus pulp. Fundamental research. 2015;(2–16):3523–3526. (In Russ.).
  7. Purente N, Li Q, Cui L, Zhang J, Peng J, He M. The influence of morphological and geographical traits on yield of Miscanthus sinensis during the first two years. Emirates Journal of Food and Agriculture. 2019;31(1):22–28.
  8. Dyshlyuk L, Babich O, Prosekov A, Ivanova S, Pavsky V, Yang Y. In vivo study of medical and biological properties of functional bakery products with the addition of pumpkin flour. Bioactive Carbohydrates and Dietary Fibre. 2017;12:20–24.
  9. Babich O, Dyshlyuk L, Noskova S, Sukhikh S, Prosekov A, Ivanova S, et al. In vivo study of the potential of the carbohydrate-mineral complex from pine nut shells as an ingredient of functional food products. Bioactive Carbohydrates and Dietary Fibre. 2019;18.
  10. Voronova MI, Surov OV, Rubleva NV, Kochkina NE, Prusova SM, Gismatulina YuA, et al. Properties of nanocrystalline cellulose obtained from celluloses of annual plants. Liquid Crystals and their Application. 2017;17(4):97–105. (In Russ.).
  11. Baibakova OV, Skiba EA. Biotechnological view of ethanol biosynthesis from miscanthus. Vavilov Journal of Genetics and Breeding. 2014;18(3):564–571. (In Russ.).
  12. Brosse N, Dufour A, Meng X, Sun Q, Ragauskas A. Miscanthus: A fast-growing crop for biofuels and chemicals production. Biofuels, Bioproducts and Biorefining. 2012;6(5):580–598.
  13. Tamura K-I, Uwatoko N, Yamashita H, Fujimori M, Akiyama Y, Shoji A, et al. Discovery of natural interspecific hybrids between Miscanthus sacchariflorus and Miscanthus sinensis in southern Japan: Morphological characterization genetic structure and origin. Bioenergy Research. 2016;9(1):315–325.
  14. Denisova MN, Pavlov IN. Sposob polucheniya tsellyulozy mnogokratnoy varkoy lekgovozobnovlyaemogo syrʹya v gidrotropnom rastvore [A new method of obtaining cellulose by multiple boiling of light-renewable raw materials in a hydrotropic solution]. Polzunovskiy Vestnik. 2015;(4–2):131–134. (In Russ.).
  15. Babich OO, Krieger OV, Chupakhin EG, Kozlova OV. Miscanthus plants processing in fuel, energy, chemical, and microbiological industries. Foods and Raw Materials. 2019;7(2):403–411.
  16. Kriger O, Budenkova E, Babich O, Suhih S, Patyukov N, Masyutin Ya, et al. The process of producing bioethanol from delignified cellulose isolated from plants of the miscanthus genus. Bioengineering. 2020;7(2).
  17. Serba EM, Tadzhibova PYu, Rimareva LV, Overchenko MB, Ignatova NI, Volkova GS. Bioconversion of soy under the influence of Aspergillus oryzae strains producing hydrolytic enzymes. Foods and Raw Materials. 2021;9(1):52–58.
How to quote?
Kriger OV, Babich OO, Dolganyuk VF, Kozlova OV, Sukhikh SA, Larichev TA. Bioethanol Production from Miscanthus sinensis Cellulose by Bioconversion. Food Processing: Techniques and Technology. 2021;51(2):387–394. https://
About journal