Abstract

Introduction. Fermentative processing of plant raw materials is traditionally carried out using native (epiphytic) microflora, which is located on the surface and represented by lactic acid microorganisms. During this process, the carbohydrates in the raw material are metabolized into lactic acid. This process does not always result in optimal product quality as the raw material often lacks carbohydrates, the optimal conditions for the development of the target microflora are hard to achieve, the microflora might be inhibited by contaminants, etc. Lactic acid microbial consortia can act as a good alternative to spontaneous fermentation of cabbage as this method creates good conditions for the microbial synergistic interaction. Such fermentation process can be controlled by adjusting the carbohydrate composition of the substrate. The research objective was to develop an analytical approach to determine the minimum required degree of change in the native carbohydrate composition of substrate that would ensure the synergy of lactic acid microorganisms.
Study objects and methods. The fermentation process was performed using white cabbage of Slava variety and such strains of lactic acid microorganisms as Lactobacillus casei VCM 536, Lactobacillus plantarum VCM B-578, and Lactobacillus brevis VCM B-1309, as well as their paired consortia. The raw material was subjected to grinding, and the epiphytic microflora was removed to create optimal conditions for the development of the lactic acid microflora.
Results and discussion. The study made it possible to define the dynamics of carbohydrate fermentation in white cabbage by various strains of lactic bacteria and their paired consortia during processing. Mathematical models helped to describe the dynamics of glucose and fructose fermentation. The experiment also demonstrated the changes that occurred in the interaction within the paired consortia during fermentation. The paper introduces a new approach to determining the minimum required degree of change in the native carbohydrate composition required to ensure synergy of lactic acid microorganisms in paired consortia.
Conclusion. The research defined the necessary amounts of carbohydrate needed to shift the integral factor of mutual influence towards sustainable synergy for three paired consortia. Consortium L. brevis + L. plantarum + 3.65 g/100 g of fructose proved to be the optimal variant for industrial production of sauerkraut from white cabbage of Slava variety. The developed approach can improve the existing industrial technologies of fermentation and create new ones.

Keywords

Fermentation, white cabbage, lactic acid microorganisms, consortia, model medium, carbohydrate digestion

REFERENCES

1. Rekomendatsii po ratsionalʹnym normam potrebleniya pishchevykh produktov, otvechayushchikh sovremennym trebovaniyam zdorovogo pitaniya [Recommended rational norms of food consumption that meet modern requirements for a healthy diet] [Internet]. [cited 2020 Oct 02]. Available from: https://www.garant.ru/products/ipo/prime/doc/71385784/.
2. Guizani N, Mothershaw A. Fermentation. In: Hui YH, Culbertson JD, editors. Handbook of food science, technology and engineering. Volume 2. Boca Raton: CRC Press; 2006. pp. 63.
3. Sinkha NK. Nastolʹnaya kniga proizvoditelya i pererabotchika plodoovoshchnoy produktsii [Handbook of the producer and processor of fruit and vegetable products]. St. Petersburg: Professiya; 2014. 896 p. (In Russ.).
4. Belokurova ES, Ivanchenko OB. Biotekhnologiya produktov rastitelʹnogo proiskhozhdeniya [Biotechnology of plant products]. St. Petersburg: Lanʹ; 2019. 232 p. (In Russ.).
5. Saravacos G, Kostaropoulos AE. Design of food processes and food processing plants. In: Saravacos G, Kostaropoulos AE, editors. Handbook of food processing equipment. Cham: Springer; 2016. pp. 1–50. https://doi.org/10.1007/978-3-319-25020-5_1.
6. Posokina NE, Lyalina OYu, Zakharova AI, Shishlova ES, Tereshonok VI. Scientifically-based approaches to the process vegetable fermentation and advantages use of bacterial starter cultures. Vegetable Crops of Russia. 2018;43(5):77–80. (In Russ.). https://doi.org/10.18619/2072-9146-2018-5-77-80.
7. Caplice E, Fitzgerald GF. Food fermentations: Role of microorganisms in food production and preservation. International Journal of Food Microbiology. 1999;50(1–2):131–149. https://doi.org/10.1016/s0168-1605(99)00082-3.
8. Bhalla TC, Savitri. Yeasts and traditional fermented foods and beverages. In: Satyanarayana T, Kunze G, editors. Yeast diversity in human welfare. Singapore: Springer; 2017. pp. 53–82. https://doi.org/10.1007/978-981-10-2621-8_3.
9. Paramithiotis S. Lactic acid fermentation of fruits and vegetables. Boca Raton: CRC Press; 2017. 312 p. https://doi.org/10.1201/9781315370378.
10. Guizani N, Mothershaw A. Chapter 9. Fermentation as a method for food preservation. In: Rahman MS, editor. Handbook of food preservation. Boca Raton: CRC Press; 2007. https://doi.org/10.1201/9781420017373.
11. Atrih A, Rekhif N, Michel M, Lefebvre G. Detection of bacteriocins produced by Lactobacillus plantarum strains isolated from different foods. Microbios. 1993;75(303):117–123.
12. Harris LJ, Fleming HP, Klaenhammer TR. Characterization of two nisin-producing Lactococcus lactis subsp. lactis strains isolated from a commercial sauerkraut fermentation. Applied and Environmental Microbiology. 1992;58(5):1477–1483. https://doi.org/10.1128/aem.58.5.1477-1483.1992.
13. Hutkins RW. Microbiology and technology of fermented foods. Wiley-Blackwell; 2006. 473 p.
14. McFeeters RF. Fermentation microorganisms and flavor changes in fermented foods. Journal of Food Science. 2004;69(1):FMS35–FMS37.
15. Salminen S, von Wright A, Ouwehand A. Lactic acid bacteria: microbiological and functional aspects. 3rd edition. Boca Raton: CRC Press; 2004. 656 p. https://doi.org/10.1201/9780824752033.
16. Drider D, Fimland G, Hechard Y, McMullen LM, Prévost H. The continuing story of class IIa bacteriocins. Microbiology and Molecular Biology Reviews. 2006;70(2):564–582. https://doi.org/10.1128/MMBR.00016-05.
17. Leroy F, De Vuyst L. Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends in Food Science and Technology. 2004;15(2):67–78. https://doi.org/10.1016/j.tifs.2003.09.004.
18. Gokulan K, Khare S, Cerniglia C. Metabolic pathways. Production of secondary metabolites of bacteria. In: Batt CA, Tortorello ML, editors. Encyclopedia of food microbiology. Second Edition. Academic Press; 2014. pp. 561–569. https://doi.org/10.1016/B978-0-12-384730-0.00203-2.