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The present paper features processes of serum biotransformation. The serum was obtained from triticale extract and pea flour after protein concentrates of increased biological value had been extracted. The research objective was to obtain microbial and vegetable feed concentrates by using a composition of Saccharomyces cerevisiae121 yeast and the yeast-like fungus Geotrichumcandidum 977. The mass fraction of protein in the two-component composites was 75–80% of the dry matter. The score of the first and the second limiting amino acids (lysine and threonine) equaled 103–113%, and that of the third acid (sulfur- containing) was 71–72%. The chemical composition of the composites corresponded to the ‘Concentrates’ group; the values of their functional and technological properties were typical of concentrates from other types of grain crops. The study revealed some cultures that are able to actively develop in serum, which is a secondary product of processing the extract after protein isolation. A symbiotic ferment was prepared from the fungus Geotrichumcandidum 977 and the yeast Saccharomyces cerevisiae 121, which ensures the growth of biomass in a carbohydrate- and nitrogen-containing medium. Proteins were isolated under the action of amylase, glucoamylase, cellulose, and xylanase. The amount of high-molecular compounds (dextrins) and trioses (raffinose) released from the interaction with protein and non-starch polysaccharides decreased 2–4 times in the solution. The amount of glucose, disaccharides, xylose, and galactose increased 2–10 times, compared with the original extracts. The serum remaining after the removal of the main mass of the protein was enriched with low molecular weight mono- and oligosaccharides, which positively affected the growth of microorganisms. The mass fraction of proteins in the microbial-vegetable composite obtained from the extract with the triticale proteins and pea flour ratio of 1:5 was 15% higher than at the ratio of 1:3. Microbial and vegetable concentrates with a mass fraction of protein of 55.8–75.1% of dry matter can be used in fodder production as a protein-carbohydrate additive. Protein composites made of protein triticale and peas with a complementary amino acid composition can improve the biological value and performance of food products.
, pea flour
, protein concentrate
, microbial and vegetable concentrate
, biological value
- Martinchik AN. Fiziologiya pitaniya [Physiology of nutrition]. Moscow: Academia; 2013. 236 p. (In Russ.).
- Kompantsev DV, Popov AV, Privalov IM, Stepanova EF. Protein isolates from vegetable raw materials: An overview of the current state and prospects of development of analysis technology of protein isolates from vegetable raw materials. Modern problems of science and education. 2016;(1):58. (In Russ.).
- Proteins: new in production technology and possibility of use. Compound feeds. 2017;(10):59–62. (In Russ.).
- Kudinov PI, Schekoldina TV, Slizkaya AS. Current status and structure of vegetable protein world resources. News institutes of higher Education. Food technology. 2012;329–330(5–6):7–10. (In Russ.).
- Domoroshchenkova ML, Lishaeva LN. Some aspects of manufacture and formation of the market of soy fibers at the present stage. Food industry. 2010;(2):32–39. (In Russ.).
- Andreev NR, Kolpakova VV, Goldstein VG. To the question of profound triticale grain processing. Food industry. 2018;(9):30–33. (In Russ.).
- Valeeva RT, Mukhachev SG, Nuretdinova EhI, Shurbina MYu, Kashapova AI. Issledovanie protsessov rosta spirtovykh i kormovykh drozhzhey na sernokislotnykh gidrolizatakh rastitelʹnogo syrʹya. Chastʹ 2. Issledovanie protsessov rosta kormovykh drozhzhey na sernistokislotnykh gidrolizatakh smesi pshenichnoy solomy i otrubey [Growth processes of alcoholic and fodder yeasts in sulfuric acid hydrolysates of plant raw materials. Part 2. Growth processes of fodder yeasts on sulfurous acid hydrolysates of wheat straw and bran mixture]. Bulletin of the Technological University. 2014;17(20):156–158. (In Russ.).
- Hrapova AV, Soprunova OB. The screening new strains of yeast for reception of fodder protein. Izvestia of Samara Scientific Center of the Russian Academy of Sciences. 2011;13(5–3):210–214. (In Russ.).
- Olaleye ON, Omotayo MA, Abdus S, Olanlege A-LO. Cellulase and Biomass Production from Sorghum (Sorghum guineense) Waste by Trichoderma longibrachiatum and Aspergillus terreus. Journal of Microbiology Research. 2015;5(6):169–174. DOI: https://doi.org/10.5923/j.microbiology.20150506.01.
- Sibtain A, Ghulam M, Muhammad A, Muhammad IR. Fungal Biomass Protein Production from Trichoderma harzianumUsing Rice Polishing. BioMed Research International. 2017;2017. DOI: https://doi.org/10.1155/2017/6232793.
- Shahzad MA, Nawaz H, Rajoka MI, Sarwar M, Sultan JI, Nisa M, et al. Use of Aspergillus terreus for microbial biomass production and its biological evaluation in broiler chicks; 2011; Singapoore. Singapoore: IACSIT Press; 2011;9:255–260.
- Shahzad MA, Rajoka MI. Single cell protein production from Aspergillus terreusand its evaluation in broiler chick. International Journal of Bioscience, Biochemistry and Bioinformatics. 2011;1(2):137–141. DOI: https://doi.org/10.7763/ IJBBB.2011.V1.25.
- Khusid SB, Gneush AN, Nesterenko EE. Sunflower husks as a source of functional feed additives. Scientific Journal of KubSAU. 2015;107(3):3–14. (In Russ.).
- Andreev NR, Kolpakova VV, Goldstein VG, Kravchenko IK, Ulanova RV, Gulakova VA, et al. Utilization of secondary tricticale processing products with production of fodder microbial-vegetative concentrate for pond fish. South of Russia: ecology, development. 2017;12(4):90–104. (In Russ.). DOI: https://doi.org/10.18470/1992-1098-2017-4-90-104.
- Lukin ND, Ulanova RV, Kravchenko IK, Kolpakova VV, Goldstein VG. Bioconversion if secondary products of grain processing of triticale on starch using the Pleurotus Ostreatus Mushroom. Chemistry of plant raw material. 2018;(4):225–234. (In Russ.). DOI: https://doi.org/10.14258/jcprm.2018043993.
- Kolpakova VV, Chumikina LV, Arabova LI, Lukin DN, Topunov AF, Titov EI. Functional technological properties and electrophoretic composition of modified wheat gluten. Foods and Raw Materials. 2016;4(2):48–57. DOI: https://doi. org/10.21179/2308-4057-2016-2-48-57.
- Dietary protein quality evaluation in human nutrition: Report of an FAO Expert Consultation. Rome: Food and Agriculture Organization of the United Nations; 2013. 66 p.
- Tutelʹyan VA. Khimicheskiy sostav i kaloriynostʹ rossiyskikh produktov pitaniya [Chemical composition and caloric content of Russian food]. Moscow: DeLi plus; 2012. 284 p. (In Russ.).
- Abramov IA, Yeliseeva NYe, Kolpakova VV, Piskun TI. Amaranth: a chemical compound, biochemical properties and ways of processing. Storage and processing of farm products. 2011;(6):44–48. (In Russ.).
- Kolpakova VV, Lukin DN, Chumikina LV, Shevyakova LV. Chemical composition and functional properties of rice protein concentrates. Proceedings of the Voronezh State University of Engineering Technologies. 2015;66(4):120–124. (In Russ.). DOI: https://doi.org/10.20914/2310-1202-2015-4-120-124.
- Vasilʹev AV, Zaytseva LV, Kolpakova VV, Chumikina LV. Gidroliz sukhoy pshenichnoy kleykoviny raznogo kachestva s primeneniem ehkzo- i ehndoproteinaz [Hydrolysis of dry wheat gluten of different quality with the use of exo-and endoproteinases]. Storage and processing of farm products. 2009;(8):38–39. (In Russ.).
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Kolpakova VV, Ulanova RV, Kulikov DS, Gulakova VA, Kadieva AT. Grain Composites with a Complementary Amino
Acid Composition in Food and Fodder. Food Processing: Techniques and Technology. 2019;49(2):301–311. (In Russ.). DOI: https://doi.