Affiliation
a Kemerovo Institute of Food Science and Technology
Copyright ©Glazunova et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0. (
http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
Abstract
The literature review allowed to select the glucose isomerase producers such as Lactobacillus brevis B-3, Streptomyces griseofuscus AS 1318 and Streptomyces wedmorensis AC 980. For the selection of a suitable strain glucose-isomerase activity, growth rate, biomass, dry matter content in the culture liquid, titratable acidity were determined. Based on the results of the experiments, the strain of Streptomyces griseofuscus differing from the analogues in more optimal features, was selected. The task of further study was to select the cultivation parameters. The effects of cultivation temperature, medium active acidity on the growth rate of the biomass of Streptomyces griseofuscus AS 1318 was analyzed. The optimum ratios of carbon sources and concentrations of Mg
2+ and Co
2+ for the glucose isomerase producer were determined.
Keywords
Lactose,
lactulose,
glucose isomerase,
biotransformation
REFERENCES
- Butova, L.I. Vozmozhnosti korrekcii narusheniya kishechnogo mikrobiocenoza laktulozoy / L.I. Butorova, A.V. Kalinin // Rossiyskiy zhurnal gastroenterologii, gepatologii, koloproktologii. – 2001. – № 1. – S. 79–83.
- Gracheva, I.M. Biotehnologiya biologicheski aktivnyh veschestv / I.M. Gracheva, L.A. Ivanova. – M.: Izd-vo NPO «Elevar», 2006. – 453 s.
- Gracheva, I.M. Laboratornyy praktikum po tehnologii fermentnyh preparatov / I.M. Gracheva. – M.: Izd-vo «Legkaya i pischevaya promyshlennost'», 1982. – 240 s.
- Primenenie fermentativnogo gidroliza laktozy / N.S. Donskoy, A.D. Lodygin, A.G. Vardanyan i dr. // Molochnaya promyshlennost'. – 2008. – № 11. – S. 44–45.
- Laktoza i ee proizvodnye / B.M. Sinel'nikov, A.G. Hramcov, I.E. Evdokimov i dr. – SPb.: Izd-vo «Professiya», 2007. – 767 s.
- Hramcov, A.G. Biotransformaciya laktozy v laktulozu / A.G. Hramcov, S.A. Ryabceva, V.K. Topalov // Sbornik nauchnyh trudov SevKavGTU. – 2007. – № 3. – S. 19–21. Seriya «Prodovol'stvie».
- Ispol'zovanie laktozy i ee proizvodnyh / A.G. Hramcov, I.A. Evdokimova, S.A. Ryabceva i dr. // Molochnaya promyshlennost'. – 2006. – № 6. – S. 50–52.
- Bhosale, S.H. Molecular and industrial aspects of glucose isomerase / S.H. Bhosale, M.V. Rao, V.V. Deshpande // Microbiol. rev. – 1996. – Vol. 60, № 2. – P. 280–300.
- Drazic, M. Isomerization of glucose to fructose using microbial enzymes / M. Drazic, Z. Golubic, S. Czimek // Period. biol. – 1980. – Vol. 82. – R. 481–484.
- Mlichova, Z. Current trends of β-galactosidase application in food technology / Z. Mlichova, M. Rosensberg // Journal of Food and Nutrition Research. – 2006. – Vol. 45, № 2. – P. 47–54.
- Lactose malabsorption and intolerance in the elderly / M. Di Stefano, G. Veneto, S. Malservisi et al. // Scand. J. Gastroenterol. – 2001. – № 36. – P. 1274–1278.
