Affiliation
a Kemerovo Institute of Food Science and Technology(University)
Copyright ©Shishin et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0. (
http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
Abstract
The intestinal microbiota plays an important role in the normal functioning of the intestine and maintaining the health of the organism. Microorganisms from intestinal tracts of healthy people and cancer patients were isolated and identified. The culture and the morphological properties of microorganisms were studied on solid medium. During the study the diameter of the colonies in millimeters, color, shape, texture, structure, surface, edge contour character were determined. The nature of the bacterial growth in the liquid media (bottom, parietal or surface, with uniform medium turbidity) was analyzed. The main method of studying the morphology of the bacteria was microscopy of fixed stained preparations. It is shown that the intestinal tract of healthy people and cancer patients contains bacteria of the following genera:
Escherichia, Klebsiella, Enterobacter, Proteus, Salmonella, Shigella, Citrobacter, Serratia, Pseudomonas, Staphylococcus, Micrococcus, Peptococcus, Sarcina, Enterococcus, Streptococcus, Peptostreptococcus, Actinomyces, Neisseria, Acinetobacter, Moraxella, Bacillus, Bacteroides, Fusobacterium, Bifidobacterium, Eubacterium, Clostridium, Campylobacter, Helicobacter, Leptotrichia, Prevotella. Also the following fungi were isolated from human intestinal tract: Candida and Cryptococcus. The results indicate that the following microorganisms show the maximum antimicrobial activity:
Bifidobacterium bifidum (the diameter of test culture growth inhibition zones is from 28.9 mm to 37.0 mm), Bifidobacterium breve (the diameter of inhibition zones - from 26.8mm to 35.6 mm),
Lactobacillus spp . (the diameter of inhibition zones - from 24.9 mm to 38.2 mm),
Micrococcus spp . (the diameter of inhibition zones - from 25.2 mm to 36.7 mm),
Streptococcus agalactiae (the diameter of inhibition zones - from 27.6 mm up to 38.4 mm).
Keywords
Intestinal tract,
bacteria,
morphological characteristics,
antimicrobial activity
REFERENCES
- Antimikrobnye peptidy mlekopitayuschih: klassifikaciya, biologicheskaya rol', perspektivy prakticheskogo primeneniya (obzornaya stat'ya) / M.S. Zharkova, D.S. Orlov, V.N. Kokryakov, O.V. Shamova // Vestnik Sankt-Peterburgskogo universiteta. Seriya 3: Biologiya. - 2014. - № 1. - S. 98-114.
- Kartuzova, O.V. Opredelenie doli aktivnosti fermentov v razlichnyh molokosvertyvayuschih preparatah / O.V. Kartuzova, A.Yu. Prosekov, O.O. Shishko, L.K. Asyakina, O.O. Babich, M.I. Zimina, S.Yu. Garmashov, O.E. Bushueva // Fundamental'nye i prikladnye issledovaniya v sovremennom mire. - 2015. - № 10-4. - S. 107-110.
- Besednova, N.N. Immunokorrektory // Zhurnal mikrobiologii, epidemiologii i immunobiologii. - 2006. - № S3. - S. 111-117.
- Grach, A.A. Rol' al'ternativnyh mehanizmov udlineniya telomer v kancerogeneze i perspektivy ispol'zovaniya antitelomeraznyh sredstv v lechenii zlokachestvennyh opuholey / A.A. Grach // Citologiya. - 2011. - T. 53. - № 10. - S. 759-771.
- Delyagin, V.M. Himio-i radiotoksicheskie porazheniya serdca u detey i podrostkov s onkogematologicheskimi zabolevaniyami / V.M. Delyagin, Yu.V. Demidova, E.A. Tihomirova // Pediatriya. Prilozhenie k zhurnalu Consilium Medicum. - 2014. - № 2. - S. 49-52.
- Issledovanie processa kompleksoobrazovaniya rekombinantnyh hsp70 cheloveka s opuholeassociirovannymi peptidami / V.A. Chernikov, N.V. Gorohovec, L.V. Savvateeva, S.E. Severin // Biomedicinskaya himiya. - 2012. - T. 58. - № 6. - S. 651-661.
- RNKaza s protivoopuholevym deystviem (binaza) vyzyvaet izmenenie kletochnoy pronicaemosti / E.A. KabreraFuentes, P.V. Zelenihin, A.I. Kolpakov, O.N. Il'inskaya // Kletochnaya transplantologiya i tkanevaya inzheneriya. - 2012. - T. VII. - № 3. - S. 72-76.
- Klebanov, G.I. Antioksidantnaya aktivnost' syvorotki krovi / G.I. Klebanov, Yu.O. Teselkin, I.V. Babenkova // Vestnik RAMN. - 1999. - № 2. - S. 15-22.
- Korolenko, T.A. Cistatiny - biologicheskaya rol' i narusheniya v patologii / T.A. Korolenko // Vestnik Rossiyskoy akademii medicinskih nauk. - 2008. - № 4. - S. 43- 45.
- Praktikum po mikrobiologii / pod red. prof. N.S. Egorova. - M.: Izd-vo MGU, 1976. - S. 308.
