Affiliation
a The Gorbatov's All-Russian Meat Research Institute (VNIIMP)
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Abstract
Application of morphometry makes it possible to widen the results of histological assay. The main stages in the quantitative study using image analysis system are reading the image from a microscope with a connected photo-video camera, its transfer to a comput- er, storing, correction of the computer program, selection of the object for morphometry, measurements, statistical processing and analysis of data, presentation of the results in tabular or graphical forms. Image adjustment includes the following steps: correction of contrast, brightness and color; noise reduction; smoothing; sharpening, and details highlighting, correction of light and white balance. The correct method of sample preparation and the choice of a special method of staining of the studied components is a determining condition for a successful automatic analysis. They are Sudan III, IV or Oil Red O, for fat components staining, Lugol solution for starch, immuno-histo-chemical methods for vegetable proteins. Automatic measurement mode reduces the time of research signifi- cantly, but requires active intervention of the operator for manual correction of operations implemented by a computer. The meas- urements are carried out with the following parameters: area (including inner area and perimeter), dimensions (diameter, chord, size, length, width), shape dimensions (form factor, circularity, elongation), characterizing the position and orientation of objects (the X and the Y, direction), or optical densitometry (brightness, optical density). The application of image analysis system is useful when carrying out a quantitative microstructure assay in accordance with GOST 32224-2013 "Meat and meat products for child nutrition. Method for determination of bone particle size", and GOST R 54047-2010 "Meat and meat products. Method of dispersion determi- nation". The use of the latest software allows increasing productivity and obtaining high quality results quickly.
Keywords
Meat,
meat products,
histology assay,
morphometry,
computer system of image analysis
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