ISSN 2074-9414 (Print),
ISSN 2313-1748 (Online)

Properties of Lactic Acid Microorganisms: Long-Term Preservation Methods

Among the relevant studies on lactic acid bacteria there are projects devoted to the multienzyme complexes of starter cultures, new competitive bacterial concentrates and their use in fermented functional milk products. In fermented milk production, the process of albuminolysis has a significant impact on the consistency, taste, and smell of the product. Therefore, lactic acid bacteria with high proteolytic properties are of the greatest interest for fermented milk industry. The present research features long- term methods for preservation of the properties of lactic acid microorganisms. The experiment defined the regime parameters of combined starter lyophilisation. The results prove that fermented milk production requires high-quality starter strains. The authors developed a long-term method for preservation of properties of particular strains of lactic acid microorganisms. The method presupposes freeze-drying with the following parameters: freezing temperature – minus 25°C in a protective 5%-glycerol medium (90 minutes); the drying temperature – 30°C (6 hours); refrigerating load – 5.45 kW/m²; residual pressure – 0.6‒0.8 kPa, bed depth – 2 mm. The authors also developed the necessary documentation (No. 9225-096-02054145-2013), procedures, and formula of the fermented milk product with a combined direct application starter.
Lactic acid bacteria, lactic fermentation, strains, lactic acid, milk acid, dairy products, multienzyme complex
  1. Kauhcheshvili N.E., Haritonov A.Yu., Sorokina N.P., and Smirnov E.A. Vacuum drying of the bacterial concentrates andlactic acid bacteria starter cultures. Dairy Industry, 2011, no. 5, pp. 30–31. (In Russ.).
  2. Frolova M.D. Some special aspects of the lyophilized starter cultures development. Dairy Industry, 2008, no. 6, pp. 30–71. (In Russ.).
  3. Settanni L., Gaglio R., Guarcello R., et al. Selected lactic acid bacteria as a hurdle to the microbial spoilage of cheese: Application on a traditional raw ewes’ milk cheese. International Dairy Journal, 2013, vol. 32, no. 2, pp. 126–132. DOI: https://doi. org/10.1016/j.idairyj.2013.04.010.
  4. State Standard 3626-77. Milk and milk products. Methods for determination of moisture and dry substance. Moscow: Standartinform Publ., 2014. 11 p.
  5. Novoselova M.V. and Prosekov A.Yu. Technological options for the production of lactoferrin. Foods and Raw Materials, 2016, vol. 4, no. 1, pp. 90–101. DOI:
  6. Sukhikh S.A., Krumlikov V.Y., Evsukova A.O., and Asyakina L.K. Formation and study of symbiotic consortium of lactobacilli to receive a direct application starter. Foods and Raw Materials, 2017, vol. 5, no. 1, pp. 51–62. DOI: https://doi. org/10.21179/2308-4057-2017-1-51-62.
  7. Strizhko M., Kuznetsova A., Galstyan A., et al. Development of osmotically active compositions for milk-based products with intermediate humidity. Bulletin of the International Dairy Federation, 2014, no. 472, pp. 35–40.
  8. Kriger O., Lisitsyn A., and Prosekov A. Characteristics of The Molecular Weight Distribution of The Prion Protein Fractions in Blood and Milk Processing Products. Biosciences Biotechnology Research Asia, 2016, vol. 13, no. 1, pp. 85–90. DOI:
  9. Prosekov A., Babich O., Kriger O., et al. Functional properties of the enzyme-modified protein from oat bran. Food Bioscience, 2018, vol. 24, pp. 46–49. DOI:
  10. Prosekov A., Babich O., Asukhikh S., Noskova S., and Dushlyuk L. The proteolytic activity research of the lactic acid microorganisms of different taxonomic groups. World Applied Sciences Journal, 2013, vol. 23, no. 10, pp. 1284–1290. DOI: https://
  11. Prosekov A.Yu. Theory and practice of prion protein analysis in food products. Foods and Raw Materials, 2014, vol. 2, no. 2, pp. 106–120. DOI:
  12. Perfilʹev G.D., Sorokina N.P., and Suslov N.V. Sostav mikroflory zakvasok i kontsentratov dlya syrodeliya [The composition of the microflora of starters and concentrates for cheese making]. Magazine Cheesemaking and Buttermaking, 1999, no. 3, pp. 10–13 (In Russ.).
  13. Kalinova M.V. Novaya bakterialʹnaya zakvaska “LAT YC” v syrodelii [New bacterial starter “LAT YC” in cheese making]. Proceedings of Nizhnevolzhskiy agrouniversity complex: science and higher vocational education, 2011, vol. 22, no. 2, pp. 123–127. (In Russ.).
  14. Prosekov A.Yu. and Ivanova S.A. Food security: The challenge of the present. Geoforum, 2018, vol. 91, pp. 73–77. DOI:
  15. Koroleva N.S., Pyatnitsyna I.N., and Bannikova L.A. Sposob prigotovleniya sukhikh zakvasok [A preparation method for a dry starter]. Dairy Industry, 1984, no. 5, pp. 27–29. (In Russ.).
  16. Kiselev D.A., Korneeva O.S., Motina E.A., and Shuvaev P.V. Lactic acid bacteria biosynthesis regulation and control. Fundamental research, 2012, no. 4–2, pp. 391–395. (In Russ.).
  17. Prosekov A.Yu., Mudrikova O.V., and Babich O.O. Determination of cinnamic acid by capillary zone electrophoresis using ion-pair reagents. Journal of Analytical Chemistry, 2012, vol. 67, no. 5, pp. 474–477. DOI: S1061934812030100.
  18. Sorokina N.P. Quality control of the bacterial concentrates and starter cultures. Dairy Industry, 2008, no. 2, pp. 20. (In Russ.).
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