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Home >Food Processing: Techniques and Technology > Archive > Volume 48, Issue 4, 2018 > Molecular Genetic Methods in Microbiological Control of Food Products
ISSN 2074-9414 (Print),
ISSN 2313-1748 (Online)

Molecular Genetic Methods in Microbiological Control of Food Products

Derevshchikova M. a
,
Mikhail Yu. Syromyatnikov b
,
Vasily N. Popov c
Affiliation
a Undergraduate, Voronezh State University
b Associate Professor of the Department of Genetics, Cytology and Bioengineering, Voronezh State University, candidate of biological sciences
c Head of the Department of Genetics, Cytology and Bioengineering, Voronezh State University, professor, doctor of biological sciences
Copyright ©Derevshchikova et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0. (http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
DOI: http://doi.org/10.21603/2074-9414-2018-4-87-113
Abstract
There are a number of technologies and business applications that identify nucleic acids of various microorganisms. Technologies based on DNA analysis are the most promising direction in the molecular-genetic identification of the microbiota in food substrates. The present paper is a review of various aspects of microorganism identification in food substrates, their advantages and disadvantages. It features modern regulatory, scientific, and methodological sources, as well as patented solutions. The authors pay considerable attention to the classical methods and describe the use of polymerase chain reaction (PCR) in microbiota analysis. Then, they trace the development of next-generation sequencing (NGS) of DNA and how it can be used to identify pathogens in food substrates. So far, NGS proves to be the most advantageous method that identifies prokaryotic and eukaryotic microorganisms, as well as pathogens.
Keywords
Next-generation sequencing (NGS), molecular genetic methods for identifying microorganisms, control, DNA, microbiota
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