Abstract

The development of the Antarctic krill fishery is a promising direction of Russian food industry. Euphausia superba is the largest source of animal protein in the global oceans. According to the Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR), the year of 2021 saw a steady increase in the global catch of krill. The Government of the Russian Federation approved a program for the development of the oceanic fishery for crustaceans. The assessment of its biological safety is of particular interest because the extraction of this raw material in Russia is currently undergoing a restoration process. The purpose of the study was to analyze samples of Antarctic krill E. superba for parasites and microorganisms that could affect its sanitary condition.
The study featured the microplankton of E. superba crustaceans (n = 130) caught in 2019–2020 by the Federal Agency for Fishery. The methods included an incomplete helminthological analysis, as well as compressor, microbiological, and histological studies.
The visual inspection, helminthological dissection, and compressor microscopy revealed no helminth larvae or protozoa. In a microbiological study for safety indicators according to CU TR 021/2011, EAEU TR 040/2016 and Sanitary Rules and Norms SanPiN 2.3.2.1078-01, the quantity of mesophilic aerobic and facultative anaerobic microorganisms (QMAFAnM) were did not exceed 1.0×10³ at 37 and 25°C. Opportunistic and pathogenic microorganisms were not detected. When examining cultivate for the isolation of Staphylococci, were found Cocci, but we did not identify them. When the material was sown on the Sabouraud nutrient medium at 24°C, Penicillium microscopic fungi grew on the plates in the amount of 3.0×10². Histological examination of carapace, gills, and internal organs detected no parasitological organisms, pathological inclusions, or any tissue changes.
The Antarctic krill contained no parasites and was microbiologically safe. After tests on Vibrio parahemolyticus and Listeria monocytogenes, it can be used in the food industry.

Keywords

Crustaceans, microorganisms, parasites, bacteria, fungi, safety

REFERENCES

1. Andreev MP. Antarctic krill (Euphausia superba) – the past, present and the future development of technology processing. Problems of Fisheries. 2021;22(1):5–15. (In Russ.). https://doi.org/10.36038/0234-2774-2021-22-1-5-15
2. Wang R, Song P, Li Y, Lin L. An integrated, size-structured stock assessment of Antarctic krill, Euphausia superba. Frontiers in Marine Science. 2021;8. https://doi.org/10.3389/fmars.2021.710544
3. Siegel V. Biology and ecology of Antarctic krill. Cham: Springer; 2016. 441 p. https://doi.org/10.1007/978-3-319-29279-3
4. Bandurin KV, Kasatkina SM. Development of Russian resource reserch and fishery for krill Euphausia superba: problems and prospects. Problems of Fisheries. 2021;22(2):20–26. (In Russ.). https://doi.org/10.36038/0234-2774-2021-22-2-20-26
5. Kaur K, Kortner TM, Benitez-Santana T, Burri L. Effects of Antarctic krill products on feed intake, growth performance, fillet quality, and health in salmonids. Aquaculture Nutrition. 2022;2022. https://doi.org/10.1155/2022/3170854
6. Li Y, Zeng Q-H, Liu G, Peng Z, Wang Y, Zhu Y, et al. Effects of ultrasound-assisted basic electrolyzed water (BEW) extraction on structural and functional properties of Antarctic krill (Euphausia superba) proteins. Ultrasonics Sonochemistry. 2021;71. https://doi.org/10.1016/j.ultsonch.2020.105364
7. Xie D, He F, Wang X, Wang X, Jin Q, Jin J. Diverse krill lipid fractions differentially reduce LPS-induced inflammatory markers in RAW264.7 macrophages in vitro. Foods. 2021;10(11). https://doi.org/10.3390/foods10112887
8. Li Y, Peng Z, Tan L, Zhu Y, Zhao C, Zeng Q-H, et al. Structural and functional properties of soluble Antarctic krill proteins covalently modified by rutin. Food Chemistry. 2022;379. https://doi.org/10.1016/j.foodchem.2022.132159
9. Zhang H-Y, Cao M-X, Fodjo EK, Kong C, Cai Y-Q, Shen X-S, et al. Safety of Antarctic krill (Euphausia superba) as food source: its initial fluoride toxicity study. Food Science and Technology. 2019;39(4):905–911. https://doi.org/10.1590/fst.11418
10. Fang B, Wang Z-H, Shi W-Z, Wang L-L, Chen X-Y. Study on the removal of various morphological fluorine in Antarctic krill meat by calcium salt. Modern Food Science and Technology. 2018;34(8):64–68. https://doi.org/10.13982/j.mfst.1673-9078.2018.8.010
11. Yan G, Bao Y, Tan M, Cui Q, Lu X, Zhang Y. Defluorination by Donnan Dialysis with seawater for seafood processing. Journal of Food Engineering. 2018;238:22–29. https://doi.org/10.1016/j.jfoodeng.2018.05.033
12. Xie J, Tao L, Wu Q, Bian Z, Wang M, Li Y, et al. Bioaccumulation of organochlorine pesticides in Antarctic krill (Euphausia superba): Profile, influencing factors, and mechanisms. Journal of Hazardous Materials. 2022;426. https://doi.org/10.1016/j.jhazmat.2021.128115
13. Galbán-Malagón CJ, Hernán G, Abad E, Dachs J. Persistent organic pollutants in krill from the Bellingshausen, South Scotia, and Weddell Seas. Science of the Total Environment. 2018;610–611;1487–1495. https://doi.org/10.1016/j.scitotenv.2017.08.108
14. Markham E, Brault EK, Khairy M, Robuck AR, Goebel ME, Cantwell MG, et al. Time trends of polybrominated diphenyl ethers (PBDEs) in Antarctic biota. ACS Omega. 2018;3(6):6595–6604. https://doi.org/10.1021/acsomega.8b00440
15. Sontag PT, Steinberg DK, Reinfelder JR. Patterns of total mercury and methylmercury bioaccumulation in Antarctic krill (Euphausia superba) along the West Antarctic Peninsula. Science of the Total Environment. 2019;688:174–183. https://doi.org/10.1016/j.scitotenv.2019.06.176
16. Han X, Liu D. Detection and analysis of 17 steroid hormones by ultra-high-performance liquid chromatography-electrospray ionization mass spectrometry (UHPLC-MS) in different sex and maturity stages of Antarctic krill (Euphausia superba Dana). PLoS ONE. 2019;14(3). https://doi.org/10.1371/journal.pone.0213398
17. Sytov AM. Feedback control procedure for the krill fishery. Modern problems and prospects for the development of the fishery complex: Materials of the V scientific and practical conference of young scientists with international participation; 2017; Moscow. Moscow: VNIRO; 2017. p. 259–263. (In Russ.).
18. Clarke LJ, Suter L, King R, Bissett A, Deagle BE. Antarctic krill are reservoirs for distinct southern ocean microbial communities. Frontiers in Microbiology. 2019;10. https://doi.org/10.3389/fmicb.2018.03226
19. Clarke LJ, Suter L, King R, Bissett A, Bestley S, Deagle BE. Bacterial epibiont communities of panmictic Antarctic krill are spatially structured. Molecular Ecology. 2021;30(4):1042–1052. https://doi.org/10.1111/mec.15771
20. Cleary AC, Casas MC, Durbin EG, Gómez-Gutiérrez J. Parasites in Antarctic krill guts inferred from DNA sequences. Antarctic Science. 2019;31(1):16–22. https://doi.org/10.1017/S0954102018000469
21. Gómez-Gutiérrez J, Morales-Ávila JR. Parasites and diseases. In: Siegel V, editor. Biology and ecology of Antarctic krill. Cham: Springer; 2016. pp. 351–386. https://doi.org/10.1007/978-3-319-29279-3_10
22. Seear PJ, Goodall-Copestake WP, Fleming AH, Rosato E, Tarling GA. Seasonal and spatial influences on gene expression in Antarctic krill Euphausia superba. Marine Ecology Progress Series. 2012;467:64–75. https://doi.org/10.3354/meps09947
23. Bykova VM. Antarctic krill. Moscow: VNIRO; 2001. 207 p. (In Russ.).
24. Cui X, Zhu G, Liu H, Jiang G, Wang Y, Zhu W. Diversity and function of the Antarctic krill microorganisms from Euphausia superba. Scientific Reports. 2016;6. https://doi.org/10.1038/srep36496
25. Wang F, Sheng J, Chen Y, Xu J. Microbial diversity and dominant bacteria causing spoilage during storage and processing of the Antarctic krill, Euphausia superba. Polar Biology. 2021;44(1):163–171. https://doi.org/10.1007/s00300-020-02789-x
26. Wang Y, Ma L, He J, Liu Z, Weng S, Wang L, et al. Whole genome sequencing and comparative genomic analyses of Planococcus alpniumensis MSAK28401T, a new species isolated from Antarctic krill. BMC Microbiology. 2021;21(1). https://doi.org/10.1186/s12866-021-02347-3
27. Efimochkina NR, Sedova IB, Sheveleva SA, Tutelyan VA. Toxigenic properties of mycotoxin-producing fungi. Tomsk State University Journal of Biology. 2019;(45):6–33. (In Russ.). https://doi.org/10.17223/19988591/45/1
28. Zhang S-Y, Zhao G-X, Suo S-K, Wang Y-M, Chi C-F, Wang B. Purification, identification, activity evaluation, and stability of antioxidant peptides from alcalase hydrolysate of Antarctic krill (Euphausia superba) proteins. Marine Drugs. 2021;19(6). https://doi.org/10.3390/md19060347
29. Miwa S, Kamaishi T, Matsuyama T, Hayashi T, Naganobu M. Histopathology of Antarctic krill, Euphausia superba, bearing black spots. Journal of Invertebrate Pathology. 2008;98(3):280–286. https://doi.org/10.1016/j.jip.2008.04.004
30. Shields JD. Collection techniques for the analyses of pathogens in crustaceans. Journal of Crustacean Biology. 2017;37(6):753–763. https://doi.org/10.1093/jcbiol/rux077