Аффилиация
a Федеральный Алтайский научный центр агробиотехнологий, Барнаул
b OOO «Вистерра», с. Алтайское
Все права защищены ©Кротова и др. Это статья с открытым доступом, распространяемая на условиях международной лицензии Creative Commons Attribution 4.0. (
http://creativecommons.org/licenses/by/4.0/), позволяет другим распространять, перерабатывать, исправлять и развивать произведение, даже в коммерческих целях, при условии указания автора произведения.
Аннотация
Панты – неокостеневшие рога оленей, снятые в период роста, распространенное сырье для изготовления биологически активных добавок с доказанным профилактическим и лечебным действием. Для расширения возможности применения пантовой продукции в составе функционального питания предлагается использование технологии разделения сырого панта на фракции. Цель исследования – оценить биохимический состав субстанций из пантов марала (Cervus elaphus sibiricus), полученных путем фракционирования.
Материалом исследования послужили сырые панты марала. Сырье фракционировали посредством перколяции, высокотемпературного гидролиза и субкритической экстракции. Все пробы высушивали до влажности 5–10 %. В представленных образцах определяли массовую долю органических и минеральных веществ, массовую концентрацию макро- и микроэлементов, аминокислот, жирнокислотный состав, молекулярно-массовое распределение фракций пептидов.
В кровяной и хрящевой субстанциях преобладала белковая фракция (74,29–80,59 %) высокой биологической ценности с показателями аминокислотного скора по треонину, фенилаланину, изолейцину и триптофану от 97 до 227 %. Анализ пептидных фракций показал наличие низкомолекулярных пептидов массой < 2,9 кДа с преобладающим их количеством в составе хрящевой субстанции. По минеральным компонентам показано преобладание кальция (16590,00 мг/100 г), натрия (799,10 мг/100 г), магния (319,80 мг/100 г) и цинка (6,40 мг/100 г) в составе субстанции из крови и лимфы; калия (408,60 мг/100 г) и железа (54,30 мг/100 г) в хрящевой фракции. Основу минеральной фракции составляет фосфор в количестве 14000,00 мг/100 г.
На основании данных биохимического состава субстанций из пантов марала (Cervus elaphus sibiricus), полученных путем фракционирования, при сравнительной оценке с исходным сырьем показана высокая биологическая ценность, что позволяет установить целесообразность их включения в функциональные продукты питания людей.
Ключевые слова
Марал,
субстанция,
фракционирование,
функциональный продукт,
панты,
биохимический состав,
пептиды,
аминокислоты
СПИСОК ЛИТЕРАТУРЫ
- Soetedjo NNM. The role of nutrition in various endocrine and metabolic diseases. Clinical Nutrition Open Science. 2025;62:164–188. https://doi.org/10.1016/j.nutos.2025.05.015
- Lee CD, Hardin CC, Longo DL, Ingelfinger JR. Nutrition in medicine – A new review article series. The New England Journal of Medicine. 2024;390(14):1324–1325. https://doi.org/10.1056/NEJMe2313282
- Lozada-Martinez ID, Vindas-Meza L, Castelblanco-Toro S, Salazar-Uribe JC, Anaya J. The impact of nutritional status on centenarians physical, mental, and functional health. Clinical Nutrition Open Science. 2025;60:10–20. https://doi.org/10.1016/j.nutos.2025.01.010
- Feng L, Chu Z, Quan X, Zhang Y, Yuan W, et al. Malnutrition is positively associated with cognitive decline in centenarians and oldest-old adults: A cross-sectional study. eClinicalMedicine. 2022;47:101336. https://doi.org/10.1016/j.eclinm.2022.101336
- Просеков А. Ю., Веснина А. Д., Любимова Н. А., Чекушкина Д. Ю., Михайлова Е. С. Потребительская геномика: роль в персонализации питания. Техника и технология пищевых производств. 2025. Т. 55. № 2. С. 400–415. https://doi.org/10.21603/2074-9414-2025-2-2582
- Yiğit A, Bielska P, Cais-Sokolińska D, Samur G. Whey proteins as a functional food: Health effects, functional properties, and applications in food. Journal of the American Nutrition Association. 2023;42(8):758–768. https://doi.org/10.1080/27697061.2023.2169208
- Gupta A, Sanwal N, Bareen MA, Barua S, Sharma N, et al. Trends in functional beverages: Functional ingredients, processing technologies, stability, health benefits, and consumer perspective. Food Research International. 2023;170:113046. https://doi.org/10.1016/j.foodres.2023.113046
- Berry CW, Murray B, Kenney WL. Scientific basis for a milk permeate-based sports drink – A critical review. International Dairy Journal. 2022;127:105296. https://doi.org/10.1016/j.idairyj.2021.105296
- Chakrabarti S, Guha S, Majumder K. Food-derived bioactive peptides: Production, biological activities, opportunities and challenges. Nutrients. 2018;10(11):1738. https://doi.org/10.3390/NU10111738
- Wang M, Zhou Z, Wei Y, He R, Yang J, et al. Dissecting the mechanisms of velvet antler extract against diabetic osteoporosis via network pharmacology and proteomics. Journal of Ethnopharmacology. 2025;341:119334. https://doi.org/10.1016/j.jep.2025.119334
- Ding C, Hao M, Ma S, Zhang Y, Yang J, et al. Identification of peptides with antioxidant, anti-lipoxygenase, anti-xanthine oxidase and anti-tyrosinase activities from velvet antler blood. LWT. 2022;168:113889. https://doi.org/10.1016/j.lwt.2022.113889
- Cao T-Q, An H-X, Ma R-J, Dai K-Y, Ji H-Y, et al. Structural characteristics of a low molecular weight velvet antler protein and the anti-tumor activity on S180 tumor-bearing mice. Bioorganic Chemistry. 2023;131:106304. https://doi.org/10.1016/j.bioorg.2022.106304
- Сатаева Ж. И., Жетимкаринов Е. Д. Напитки профилактического назначения на основе порошков панты оленей. Norwegian Journal of development of the International Science. 2024. № 140. С. 4–8. https://doi.org/10.5281/zenodo.13768163
- Казанцев Д. А., Растопшина Л. В. Характеристика стада маралов алтае-саянской породы в СПК ПЗ «Абайский». Вестник Алтайского государственного аграрного университета. 2021. № 5. С. 88–92.
- Sui Z, Sun H, Weng Y, Zhang X, Sun M, et al. Quantitative proteomics analysis of deer antlerogenic periosteal cells reveals potential bioactive factors in velvet antlers. Journal of Chromatography A. 2020;1609:460496. https://doi.org/10.1016/j.chroma.2019.460496
- Chen Y, Zhang Z, Jin W, Li Z, Bao C, et al. Integrative analyses of antler cartilage transcriptome and proteome of gansu red deer (Cervus elaphus kansuensis) at different growth stages. Animals. 2022;12(7):934. https://doi.org/10.3390/ani12070934
- Yao B, Zhou Z, Zhang M, Leng X, Zhao D. Investigating the molecular control of deer antler extract on articular cartilage. Journal of orthopaedic Surgary and Research. 2021;16:8. https://doi.org/10.1186/s13018-020-02148-w
- Коростелева Н. И., Кондрашкова И. С., Рудишина Н. М., Камардина И. А. Биометрия в животноводстве. Барнаул: АГАУ; 2009. 210 с.
- Singh RR, Khanna PP, Singh AK, Goyal SP. Elemental characterization of antlers of variours deer species using X-Ray fluorescence (XRF): A tool for forensic examination. Forensic Science International. 2022;332:111172. https://doi.org/10.1016/j.forsciint.2022.111172
- Orassay A, Sadvokassova D, Berdigaliyev A, Sagintayev A, Myrzagali S, et al. Deer antler extract: Pharmacology, rehabilitation and sports medicine applications. Pharmacologycal Research – Modern Chinese Medicine. 2024;10:100316. https://doi.org/10.1016/j.prmcm.2023.100316
- Jeon B, Kim S, Lee S, Park P, Sung S, et al. Effect of antler growth period on the chemical composition of velvet antler in sika deer (Cervus nippon). Mammalian Biology. 2009;74(5):374–380. https://doi.org/10.1016/j.mambio.2008.07.005
- Jang DW, Ameer K, Oh JH, Park MK. Optimization and pretreatment for hot water extraction of Korean deer (Cervus canadensis erxleben) velvet antlers. Journal of microbiology and biotechnology. 2020;30(8):1116–1123. https://doi.org/10.4014/JMB.2004.04009
- Shi M, Li T, Zhao Y, He Z, Zong Y, et al. Comparative studies on the chemical composition and pharmacological effects of vinegar-processed antler glue modified from Lei Gong Pao Zhi Lun and traditional water-processed antler glue. Journal of Ethnopharmacology. 2024;321:117508. https://doi.org/10.1016/j.jep.2023.117508
- Widyowati R, Suciati S, Haryadi DM, Chang H-I, Suryawan IPGN, et al. The effect of Rusa unicolor antler deer extracts from East Kalimantan in bone turnover cell models. Turkish Journal of Pharmaceutical Sciences. 2020;17(4):440–445. https://doi.org/10.4274/tjps.galenos.2019.57805
- Sui Z, Zhang L, Huo Y, Yukui Z. Bioactive components of velvet antlers and their pharmacological properties. Journal of Pharmaceutical and Biomedical Analysis. 2014;87:229–240. https://doi.org/10.1016/j.jpba.2013.07.044
- López-Pedrouso M, Lorenzo JM, Landete-Castillejos T, Chonco L, Pérez-Barbería FJ, et al. Quantitative proteomic analysis of deer antler from two regenerating and mineralizing sections. Biology. 2021;10(7):679. https://doi.org/10.3390/biology10070679
- Кротова М. Г., Гришаева И. Н. Коллаген, гидролизованный из сырья маралов: технология получения и биохимический состав. Техника и технология пищевых производств. 2024. Т. 54. № 4. С. 884–896. [Krotova MG, Grishaeva IN. Collagen hydrolysed from maral raw material: Production technology and biochemical composition. Food Processing: Techniques and Technology. 2024;54(4):884–896. (In Russ.)] https://doi.org /10.21603/2074-9414-2024-4-2549
- Guo H, Zhang Q, Xin L, Zhang H, Wang S. Dietary hydroxyproline promotes collagen deposition in swim bladder through regulating biosynthesis of amino acid: In-vitro and in-vivo investigations in Nibea coibor. Aquaculture. 2023;573:739614. https://doi.org/10.1016/j.aquaculture.2023.739614
- Krishnan A, Raghu S, Eswaramoorthy R, Perumal G. Biodegradable glutamic acid loaded polycaprolactone nanofibrous scaffold for controlled dentin mineralization. Journal of Drug Delivery Science and Technology. 2025;104:106546. https://doi.org/10.1016/j.jddst.2024.106546
- Lioi M, Tengattini S, Gotti R, Bagatin F, Galliani S. Chromatographic separation by RPLC-ESI-MS of all hydroxyproline isomers for the characterization of collagens from different sources. Journal of Chromatography A. 2024;1720:464771. https://doi.org/10.1016/j.chroma.2024.464771
- Rucklidge GJ, Milne G, Bos KJ, Farquharson C, Robins SP. Deer antler does not represent a typical endochondral growth system: Immunoidentification of collagen type X but little collagen type II in growing antler tissue. Comparative Biochemistry and Physiology part B: Biochemistry and Molecular Biology. 1997;118(2):303–308. https://doi.org/10.1016/S0305-0491(97)00171-5
- Гордынец С. А., Мадзиевская Т. А., Курченко В. П. Новые тенденции в создании пищевых ингредиентов. Материалы семинара с международным участием. Минск: Издательский центр БГУ; 2021. 106 с.
- Joint WHO/FAO/UNU Expert Consultation. Protein and amino acid requirements in human nutrition. WHO Technical Report Series. 2007;(935):1–265.
- Яременко О. Б., Анохина Г. А., Бурьянов А. А. Сустав. Хрящ. Коллаген. Травма. 2020. Т. 21. № 4. С. 6–12. https://doi.org/10.22141/1608-1706.4.21.2020.212531
- Lin L, Li C, Zhang T, Xia C, Bai Q, et al. An in silico scheme for optimizing the enzymatic acquisition of natural biologically active peptides based on machine learning and virtual digestion. Analytica Chimica Acta. 2024;1298:342419. https://doi.org/10.1016/j.aca.2024.342419
- Zamudio FV, Hidalgo-Figueroa SN, Ortíz Andrade RR, Hernández Álvarez AJ, Segura Campos MR. Identification of antidiabetic peptides derived from in silico hydrolysis of three ancient grains: Amaranth, Quinoa and Chia. Food Chemistry. 022;394:133479. https://doi.org/10.1016/j.foodchem.2022.133479
- Chirinos R, Escobar-Mendoza N, Figueroa-Merma A, de Oliveira TV, Guzmán F, et al. Evaluation of the antihypertensive and antidiabetic potential of peptides from the globulin fraction of quinoa (Chenopodium quinoa) by an in silico and in vitro approach. International Journal of Food Science and Technology. 2023;58(8):4386–4396. https://doi.org/10.1111/ijfs.16544
- Pektaş AN, Korkmaz EM. Novel antimicrobial defensin peptides from different coleopteran insects (Coleoptera: Insecta): Identification, characterisation and antimicrobial properties. Journal of Asian Natural Products Research. 2025;27(8):1146–1160. https://doi.org/10.1080/10286020.2024.2448011
- Ghafoor H, Asim MN, Ibrahim MA, Ahmed S, Dengel A. CAPTURE: Comprehensive anti-cancer peptide predictor with a unique amino acid sequence encoder. Computers in Biology and Medicine. 2024;176:108538. https://doi.org/10.1016/j.compbiomed.2024.108538
- Hajigha MN, Hajikhani B, Vaezjalali M, Kafil HS, Anari RK, et al. Antiviral and antibacterial peptides: Mechanisms of action. Heliyon. 2024;10(22):e40121. https://doi.org/10.1016/j.heliyon.2024.e40121
- Смирнова А. В., Тихонов С. А. Идентификация и предиктивный анализ аминокислотных паттернов, обуславливающих потенциальную антигиперурикемическую активность пептидов. Техника и технология пищевых производств. 2024. Т. 54. № 4. С. 687–700. https://doi.org/10.21603/2074-9414-2024-4-2536
- Mottola S, Del Bene A, Mazzarella V, Cutolo R, Boccino I, et al. Sustainable ultrasound-assisted solid-phase peptide synthesis (SUS-SPPS): Less waste, more efficiency. Ultrasonics Sonochemistry. 2025;114:107257. https://doi.org/10.1016/j.Ultsonch.2025.107257
- Kohl J, Jerger S, König D, Centner C. Chapter 21 – Applications in nutrition: Sport nutrition. In: Toldrá F, Wu J, editors. Biologically Active Peptides. NY, Oxford: Academic Press; 2021. pp. 525–550. https://doi.org/10.1016/B978-0-12-821389-6.00024-8
- Chandimali N, Bak S-G, Park EH, Lim H-J, Won Y-S, et al. Bioactive peptides derived from duck products and byproducts as functional food ingredients. Journal of Functional Foods. 2024;113:105953. https://doi.org/10.1016/j.jff.2023.105953
- Asim MN, Asif T, Mehmood F, Dengel A. Peptide classification landscape: An in-depth systematic literature review on peptide types, databases, datasets, predictors architectures and performance. Computers in Biology and Medicine. 2025;188:109821. https://doi.org/10.1016/j.compbiomed.2025.109821
- Bao X, Wu J. Impact of food-derived bioactive peptides on gut function and health. Food Research International. 2021;147:110485. https://doi.org/10.1016/j.foodres.2021.110485
- Chen D, Shu Y, Chen J, Cao X. Preparation and in vitro bioactive evaluation of cashew-nut proteins hydrolysate as a potential source of anti-allergy peptides. Journal of Food Science & Technology. 2021;58:3780–3789. https://doi.org/10.1007/s13197-020-04838-z
- Suttie JM, Gluckman PD, Butler JH, Fennessy PF, Corson ID, et al. Insum-like growth factor 1 (IGF-1) antlerstimulating holmone? Endocrinology. 1985;116(2):846–848. https://doi.org/10.1210/endo-116-2-846
- Zhao L, Wang X, Zhang X, Xie Q. Purification and identification of anti-inflammatory peptides derived from simulated gastrointestinal digests of velvet antler protein (Cervus elaphus Linnaeus). Journal of Food and Drug Analisis. 2016;24(2):376–384. https://doi.org/10.1016/j.jfda.2015.10.003
- Ma S, Ding Q, Xia G, Li A, Li J, et al. Multifunctional biomaterial hydrogel loaded with antler blood peptide effectively promotes wound repair. Biomedicine & Pharmacotherapy. 2024;170:116076. https://doi.org/10.1016/j.biopha.2023.116076
- Hao M, Peng X, Sun S, Ding C, Liu W, et al. Chitosan/sodium alginate/velvet antler blood peptides hydrogel promoted wound healing by regulating PI3K/AKT/mTOR and SIRT1/NF-kB pathways. Frontiers in Pharmacology. 2022;13:913408. https://doi.org/10.3389/fphar.2022.913408
- Gómez JA, Ceacero F, Landete-Castillejos T, Gaspar-López E, García AJ, et al. Factors affecting antler investment in Iberian red deer. Animal Production Science. 2012;52:867–873. https://doi.org/10.1071/AN11316
- Shi H, Yu T, Li Z, Lu W, Zhang M, et al. Bone regeneration strategy inspired by the study of calcification behavior in deer antler. Materials Science and Engineering: C. 2015;57:67–76. https://doi.org/10.1016/j.msec.2015.07.043
- Hamid HA, Khairul Anuar MZA, Zulkifli FH. Preparation and characterization of deer velvet antler/polyvinyl alcohol (DVA/PVA) scaffold for bone tissue engineering. Materialstoday: Proceedings. 2022;51(Part 2):1332–1337. https://doi.org/10.1016/j.matpr.2021.11.377
- Sawant RC, Somkuwar SR, Luo S-Y, Kamble RB, Panhekar DY, et al. Chapter 4 – Novel extraction and characterization methods for phytochemicals. In: Pati S, Sarkar T, Lahiri D, editors. Recent Frontiers of Phytochemicals. Netherlands: Elsevier; 2023. pp. 63–84. https://doi.org/10.1016/B978-0-443-19143-5.00035-9
- Chonco L, Landete-Castillejos T, Serrano-Heras G, Pérez Serrano M, Pérez-Barbería FJ, et al. Anti-tumour activity of deer growing antlers and its potential applications in the treatment of malignant gliomas. Scientific Reports. 2021;11:42. https://doi.org/10.1038/s41598-020-79779-w
- Loftus LV, Rolle LTA, Wang B, Pienta KJ, Amend SR. Dysregulation of labile iron predisposes chemotherapy resistant cancer cells to ferroptosis. International Journal of Molecular Sciences. 2025;26(9):4193. https://doi.org/10.3390/ijms26094193
- Kim C-T, Gujral N, Ganguly A, Suh J-W, Sunwoo HH. Chondroitin sulphate extracted fromantler cartilage using high hydrostatic pressure and enzymatic hydrolysis. Biotechnology reports. 2014;4:14–20. https://doi.org/10.1016/j.btre.2014.07.004
- Collazo N, Carpena M, Nuñez-Estevez B, Otero P, Simal-Gandara J, et al. Health promoting properties of bee royal jelly: Food of the queens. Nutrients. 2021;13(2):543. https://doi.org/10.3390/nu13020543