Abstract

Introduction. Nutrition has a significant impact on human health, performance, well-being, and lifespan. The research objective was to develop formulations for functional compositions of seafood cryoconcentrates.
Study objects and methods. The study featured concentrates obtained by cryotechnology from carcasses of Pacific squid (Todarodes pacificus), muscular shell of cucumaria (Cucumaria japonica), scallop mantle (Mizuhopecten yessoensis), octopus skin (Octopus dofleini), milt of Pacific herring (Pacific herring), and Japanese kelp (Laminaria japonica), as well as their multicomponent mixes. The content of biologically active substances was determined by the spectrophotometric method. The safety and biological value were biotested on Tetrahymena pyriformis.
Results and discussion. The dry seafood concentrates had a high content of protein, minerals, and biologically active components, i.e. hexosamines, triterpene glycosides, carotenoids, nucleic acids, etc. The seafood cryoconcentrate mixes were divided into fortifying, restoring, immunomodulating, and mineral. Their chemical composition varied greatly, depending on the functional aim of the mix. For instance, the protein content varied from 28.8 to 57.6%, lipids – from 3.1 to 6.9%, carbohydrates – from 15.0 to 35.9%, minerals – from 10.9 to 22.9%, and hexosamines – from 2.2 to 5.6%. The experiments made it possible to substantiate the composition of the dry mixes. The compilation principle was based on the chemical profile of the raw material, as well as on the choice of biologically active substances. All the dry seafood concentrates showed a high relative biological value. However, the values differed, depending on the type of raw material: from 65.5% for the scallop mantle sample to 86.3% for the octopus skin sample. The difference could be explained by the chemical composition of the cryopowders and their availability to ciliate enzymes.
Conclusion. The research resulted in formulations and functional mixes of dry seafood concentrates obtained by cryotechnology. The dry seafood concentrates can be used both independently and as functional compositions. The developed mixes demonstrated rather high content of animal proteins, minerals, and biologically active substance, e.g. hexosamines, triterpene glycosides, carotenoids, nucleic acids, etc. Their functional compositions proved to have excellent sensory properties; they are biologically safe for human consumption and have a high relative biological value.

Keywords

Cryopowders, dry concentrates, seafoods, chemical composition, organoleptic properties, biological value

REFERENCES

1. Potoroko IYu, Paimulina AV, Uskova DG, Kalinina IV. Scientific and practical aspects of functional food technology. Bulletin of the South Ural State University. Series: Food and Biotechnology. 2018;6(1):49–59. (In Russ.). https://doi.org/10.14529/food180106.
2. Souissi N, Jridi M, Nasri R, Ben Slama R, Njeh M, Nasri M. Effects of the edible cuttlefish gelatin on textural, sensorial and physicochemical quality of octopus sausage. LWT – Food Science and Technology. 2016;65:18–24. https://doi.org/10.1016/j.lwt.2015.07.051.
3. Kovalev NN, Poznyakova YuM, Pertseva AD, Tun C. The composition and antioxidant enzymatic hydrolyzate of trepang muscle. Food Industry. 2016;(1):52–55. (In Russ.).
4. Severin SE, Solovʹeva GA. Praktikum po biokhimii [Biochemistry workshop]. Moscow: Lomonosov Moscow State University; 1989. 509 p. (In Russ.).
5. Bogdanov VD, Sakharova OV, Sakharova TG. Safety studies and biological value of dry concentrate holothuroidea biotesting. Scientific Journal of the Far East State Technical Fisheries University. 2016;37:93–98. (In Russ.).
6. Giménez B, Gómez-Estaca J, Alemán A, Gómez-Guillén MC, Montero MP. Physico-chemical and film forming properties of giant squid (Dosidicus gigas) gelatin. Food Hydrocolloids. 2009;23(3):585–592. https://doi.org/10.1016/j.foodhyd.2008.07.003.
7. Lisitsyn AB, Chernukha IM, Lunina OI. Modern trends in the development of the functional food industry in Russia and abroad. Theory and Practice of Meat Processing. 2018;3(1):29–45. (In Russ.). https://doi.org/10.21323/2414-438X-2018-3-1-29-45.
8. Lin L, Yang K, Zheng L, Zhao M, Sun W, Zhu Q, et al. Anti-aging effect of sea cucumber (Cucumaria frondosa) hydrolysate on fruit flies and D-galactose-induced aging mice. Journal of Functional Foods. 2018;47:11–18. https://doi.org/10.1016/j.jff.2018.05.033.
9. Sukkhown P, Jangchud K, Lorjaroenphon Y, Pirak T. Flavored-functional protein hydrolysates from enzymatic hydrolysis of dried squid by-products: Effect of drying method. Food Hydrocolloids. 2018;76:103–112. https://doi.org/10.1016/j.foodhyd.2017.01.026.
10. Zuzgina AA, Kupina NM. Chemical composition and technological characteristics of two Octopoda species from the Japan Sea. Izvestiya TINRO. 2005;142:323–328. (In Russ.).
11. Mansour MB, Balti R, Yacoubi L, Ollivier V, Chaubet F, Maaroufi RM. Primary structure and anticoagulant activity of fucoidan from the sea cucumber Holothuria polii. International Journal of Biological Macromolecules. 2019;121:1145–1153. https://doi.org/10.1016/j.ijbiomac.2018.10.129.
12. Hsu W-J, Lin M-H, Kuo T-C, Chou C-M, Mi F-L, Cheng C-H, et al. Fucoidan from Laminaria japonica exerts antitumor effects on angiogenesis and micrometastasis in triple-negative breast cancer cells. International Journal of Biological Macromolecules. 2020;149:600–608. https://doi.org/10.1016/j.ijbiomac.2020.01.256.
13. Zhang X, Jiang D, Li D, Yu C, Dong X, Qi H. Characterization of a seafood-flavoring enzymatic hydrolysate from brown alga Laminaria japonica. Journal of Food Measurement and Characterization. 2019;13(2):1185–1194. https://doi.org/10.1007/s11694-019-00034-6.
14. Ai C, Jiang P, Liu Y, Duan M, Sun X, Luo T, et al. The specific use of alginate from: Laminaria japonica by Bacteroides species determined its modulation of the Bacteroides community. Food and Function. 2019;10(7):4304–4314. https://doi.org/10.1039/C9FO00289H.
15. Li X-Y, Chen H-R, Zha X-Q, Chen S, Pan L-H, Li Q-M, et al. Prevention and possible mechanism of a purified Laminaria japonica polysaccharide on adriamycin-induced acute kidney injury in mice. International Journal of Biological Macromolecules. 2020;148:591–600. https://doi.org/10.1016/j.ijbiomac.2020.01.159.
16. Mohibbullah M, Bashir KMI, Kim S-K, Hong Y-K, Kim A, Ku S-K. Protective effects of a mixed plant extracts derived from Astragalus membranaceus and Laminaria japonica on PTU-induced hypothyroidism and liver damages. Journal of Food Biochemistry. 2019;43(7). https://doi.org/10.1111/jfbc.12853.
17. Giménez B, Alemán A, Montero P, Gómez-Guillén MC. Antioxidant and functional properties of gelatin hydrolysates obtained from skin of sole and squid. Food Chemistry. 2009;114(3):976–983. https://doi.org/10.1016/j.foodchem.2008.10.050.
18. Nam KA, You SG, Kim SM. Molecular and physical characteristics of squid (Todarodes pacificus) skin collagens and biological properties of their enzymatic hydrolysates. Journal of Food Science. 2008;73(4):C249–C255. https://doi.org/10.1111/j.1750-3841.2008.00722.x.
19. Tabakaeva OV, Tabakaev AV. Tissue carotenoid composition of the Far-East bivalve mollusk Anadara broughtonii. Chemistry of Natural Compounds. 2015;51(6):1171–1173. https://doi.org/10.1007/s10600-015-1522-2.
20. Zhong Y, Khan MA, Shahidi F. Compositional characteristics and antioxidant properties of fresh and processed sea cucumber (Cucumaria frondosa). Journal of Agricultural and Food Chemistry. 2007;55(4):1188–1192. https://doi.org/10.1021/jf063085h.
21. Durand R, Fraboulet E, Marette A, Bazinet L. Simultaneous double cationic and anionic molecule separation from herring milt hydrolysate and impact on resulting fraction bioactivities. Separation and Purification Technology. 2019;210:431–441. https://doi.org/10.1016/j.seppur.2018.08.017.
22. Kodentsova VM, Vrzhesinskaya OA. The analysis of domestic and international policy of food fortification with vitamins. Problems of Nutrition. 2016;85(2):31–50. (In Russ.).