ISSN 2074-9414 (Print),
ISSN 2313-1748 (Online)

Efficacy of Antimicrobials in Fermented Milk Storage

Abstract
The existing methods of disinfection of containers for fermented milk products proved to be ineffective. The present research featured an antimicrobial preparation based on silver, copper, and zinc nanoparticles as a disinfectant solution. The authors studied the properties of the antimicrobial in order to prove its effectiveness in processing containers to increase the shelf-life of fermented milk products. The size of the silver nanoparticles ranged from 1 to 10 nm. The nanoscale particles of copper were obtained by the method of electric explosion. It was established that the mass fraction of the active substance (active metals) in the antimicrobial was 50.1%; the mass fraction of water was 11.2%; the mass fraction of hydrogen peroxide was 1.0%. To assess the ability of the antimicrobial to increase shelf-life of fermented milk products, the authors analyzed the microbiological properties of cottage cheese. The 50-gram samples were packed in pre-treated polyethylene containers that had been cleaned with the antimicrobial. After that the samples were left for storage at minus 4 ± 1°C. The quality and the microbiological state of the cottage cheese was evaluated on days 3, 5, 7, 9, 11, and 13, which was justified by the experimental conditions and regulatory documentation. Eventually, it was established that the product contained no pathogenic bacteria, such as L. monocytogenes, Salmonella, E. coli, staphylococci, yeast, molds or any viable microbial cells. It was proved that the test sample retained its consumer properties for 13 days, while the control sample was found unusable by day 7. Thus, by using the antimicrobial, it was possible to increase the shelf life of the dairy product by more than 5 days.
Keywords
Fermented milk products, plastic packaging, antibacterial drug, antimicrobial, colloidal solution, nanoparticles
REFERENCES
  1. Weese J.S. and Prescott J.F. Assessment of laboratory and biosafety practices associated with bacterial culture in veterinaryclinics. Journal of the American Veterinary Medical Association, 2009, vol. 234, no. 3, pp. 252–258.
  2. Khmelev V.N., Shalunov A.B., Golykh R.N., and Shalunova K.V. Kompleksnoe issledovanie akusticheskoy koagulyatsii melkodispersnogo aehrozolya [Complex study of acoustic coagulation of fine aerosol]. Polzunovsky vestnik, 2010, no. 3, pp. 303–309. (In Russ.).
  3. R 4.2.2643–10. Metody laboratornykh issledovaniy i ispytaniy dezinfektsionnykh sredstv dlya otsenki ikh ehffektivnosti i bezopasnosti: rukovodstvo [R 4.2.2643–10. A guide to the methods of laboratory research and testing of disinfectants to assess their effectiveness and safety]. Moscow: Federal Center of Hygiene and Epidemiology Publ., 2011. 615 p.
  4. Igumenshchev P.A. and Zhirikhina M.S. Primenenie nanostrukturirovannykh biotsidov serii “VELT” na obʺektakh vetnadzora [Application of nanostructured biocides of the VELT series on veterinary inspection sites]. Dezinfektsiya. Antiseptika [Disinfection. Antiseptic], 2011, no. 1, pp. 42–47. (In Russ.).
  5. Gryazneva T.N., Igumenshchev P.A., and Zhirikhina M.S. Perspektivnye innovatsionnye proekty v veterinarii [Promising innovative projects in veterinary medicine]. Veterinary medicine, 2011, no. 2, pp. 21–24. (In Russ.).
  6. Mikhae M.M., Huddleston P.M., Zobitz M.E., et al. Mechanical strength of bone allografts subjected to chemical sterilization and other terminal processing methods. Journal of Biomechanics, 2008, vol. 41, no. 13, pp. 2816–2820. DOI: https://doi.org/10.1016/j. jbiomech.2008.07.012.
  7. Afinogenov G.E. Chashechnyy metod otsenki ehffektivnosti dezinfektantov i antiseptikov [Cup method for evaluating the effectiveness of disinfectants and antiseptics]. St. Petersburg: I.P. Pavlov First Saint Petersburg State Medical University Publ., 2000. 5–7 p. (In Russ.).
  8. Rozalyonok T.A. and Sidorin Yu.Yu. A comparative study of antimicrobial activity of silver clusters against variousmicroorganisms. Science Evolution, 2016, vol. 1, no. 2, pp. 85–91.
  9. Rozalyonok T.A. Comparative assessment of biocidal compositions on the basis of cluster silver. Yu.A. Ovchinnikov bulletin of biotechnology and physical and chemical biology, 2016, vol. 12, no. 2, pp. 5–11. (In Russ.).
  10. Naber J. Switching to sodium hypocholorite disinfection 11 Water Environment & Technology. Alexandria, 2003, vol. 15, no. 9, pp. 100.
  11. Kimman T.G., Smit E., and Klein M.R. Evidence–based biosafety: a review of principles and effectiveness of microbiological containment measures. Clinical Microbiology Reviews, 2008, vol. 21, no. 3, pp. 403–425. DOI: https://doi.org/10.1128/CMR.00014-08.
  12. Rozalyonok T.A. and Sidorin Yu.Yu. The use of cluster composites to give food packaging antimicrobial properties.Technology and merchandising of the innovative foodstuff, 2016, vol. 39, no. 4, pp. 84–88. (In Russ.).
  13. Rozalyonok T.A. Ehkologichnye biotsidy na osnove kompozitov klasternogo serebra dlya obrabotki bumagi/kartona [Eco-friendly biocides based on composites of cluster silver for paper and cardboard processing]. Sbornik trudov Mezhdunarodnogo simpoziuma “Innovatsii v pishchevoy biotekhnologii” [Collected Works of the International Symposium “Innovations in Food Biotechnology”]. Kemerovo, 2018, pp. 490–494.
  14. Vozmilov A.G., Bakhtyreva N.G., Mikhaylov P.M., et al. Ustroystvo dlya deratizatsii [Device for disinfestation]. Patent RF, no. 95224, 2010.
  15. Kashtanov, A. V. Izuchenie fungicidnoy aktivnosti metacida / A. V. Kashtanov // Problemy veterinarnoy sanitarii, gigieny i ekologii. – 2003. – № 115. – C. 237–241.
  16. McDonnell G. and Russell A.D. Antiseptics and Disinfectants: Activity, Action and Resistance. Clinical Microbiology Reviews, 1999, vol. 12, no. 1, pp. 147–179.
  17. Myalenko D.M. Sovershenstvovanie tekhnologii rasfasovki molochnoy produktsii putem obezzarazhivaniya potrebitelʹskoy tary impulʹsnym ulʹtrafioletovym izlucheniem. Avtoref. diss. kand. tekhn. nauk [Improving the technology of dairy products packaging by disinfecting consumer packaging with pulsed ultraviolet radiation. Cand. eng. sci. thesis]. Moscow, 2009. 177 p.
  18. MUK 4.2.2884–11. Metody mikrobiologicheskogo kontrolya obʺektov okruzhayushchey sredy i pishchevykh produktov s ispolʹzovaniem petrifilʹmov: metodicheskie ukazaniya [MUK 4.2.2884-11. Methods of microbiological control of environmental objects and food by using petrilofilms: guidelines]. Moscow: Federal Center of Hygiene and Epidemiology Publ., 2011. 24 p.
  19. Niu L., Zhang H., Wei F., et al. Corrosion inhibition of iron in acidic solutions by alkyl quaternary ammonium halides: Correlation between inhibition efficiency and molecular structure. Applied Surface Science, 2005, vol. 252, no. 5, pp. 1634–1642. DOI: https://doi.org/10.1016/j.apsusc.2005.02.134.
  20. MUK 4.2.1847–04. Metody kontrolya. Biologicheskie i mikrobiologicheskie faktory. Sanitarno-ehpidemiologicheskaya otsenka obosnovaniya srokov godnosti i usloviy khraneniya pishchevykh produktov: metodicheskie ukazaniya [MUK 4.2.1847-04. Control methods. Biological and microbiological factors. Sanitary-epidemiological assessment of the justification of shelf-life and storage conditions of food products: guidelines]. Moscow, 2004.
  21. Kashtanov A.V. Izuchenie fungitsidnoy aktivnosti metatsida. Problemy veterinarnoy sanitarii, gigieny i ehkologii [Study of the fungicidal activity of metacid. Problems of veterinary sanitation, hygiene, and ecology]. Problemy Veterinarnoi Sanitarii, Gigieny i Ecologii, 2003, no. 115, pp. 237–241. (In Russ.).
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